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444258 MMP-14 Substrate I, Fluorogenic - Calbiochem

A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity.

MMP-14 Substrate I, Fluorogenic - Calbiochem : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: MT1-MMP Substrate I, Fluorogenic, MCA-PLA-C(OMeBz)-WAR(Dpa)-NH₂

444258
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Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
444258-1MG Ampoule plast. 1 mg
Description
OverviewA specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity. (kcat/Km = 7.3 x 105 M-1s-1 for MMP-14 (MT1-MMP) and 3.6 x 104 M-1s-1 for MMP-11 at 25°C, pH 7.5).
Catalogue Number444258
Brand Family Calbiochem®
SynonymsMT1-MMP Substrate I, Fluorogenic, MCA-PLA-C(OMeBz)-WAR(Dpa)-NH₂
References
ReferencesMucha, A., et al. 1998. J. Biol. Chem. 273, 2763.
Product Information
ATP CompetitiveN
FormYellow lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₆₆H₈₂N₁₆O₁₇S
Chemical formulaC₆₆H₈₂N₁₆O₁₇S
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetMMP-14
Primary Target IC<sub>50</sub>kcat/Km = 7.3 x 105 M-1s-1 for MMP-14; .6 x 104 M-1s-1 for MMP-11
Purity≥97% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceMCA-Pro-Leu-Ala-Cys(p-OMeBz)-Trp-Ala-Arg(Dpa)-NH₂ Dpa = N³-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl, Bz = Benzyl
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
444258-1MG 04055977186550

Documentation

MMP-14 Substrate I, Fluorogenic - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

MMP-14 Substrate I, Fluorogenic - Calbiochem Certificats d'analyse

TitreNuméro de lot
444258

Références bibliographiques

Aperçu de la référence bibliographique
Mucha, A., et al. 1998. J. Biol. Chem. 273, 2763.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-March-2009 JSW
SynonymsMT1-MMP Substrate I, Fluorogenic, MCA-PLA-C(OMeBz)-WAR(Dpa)-NH₂
DescriptionA fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity (kcat/km = 730,000 M-1s-1 for MMP-14 (MT1-MMP) and 36,000 M-1s-1 for MMP-11 (ST3); pH = 7.5 at 25°C). Excitation max.: ~328 nm; emission max.: ~400 nm.
FormYellow lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₆₆H₈₂N₁₆O₁₇S
Peptide SequenceMCA-Pro-Leu-Ala-Cys(p-OMeBz)-Trp-Ala-Arg(Dpa)-NH₂ Dpa = N³-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl, Bz = Benzyl
Purity≥97% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMucha, A., et al. 1998. J. Biol. Chem. 273, 2763.