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48-630MAG MILLIPLEXMAP NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
48-630MAG
96-well plate  96-well plate
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
c-Myc FADD (Ser194) IκBα (Ser32) IKKα/β (Ser177/Ser181) NFκB (Ser536) TNFR1 HMplexLuminex xMAP
Description
Catalogue Number48-630MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEXMAP NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay
OverviewNF-κB (nuclear factor kappa B) is a transcription factor that plays a regulatory role in several physiological and pathological processes. The NF-κB pathway can be activated by numerous stimuli, including inflammatory cytokines, virus, bacteria, UV radiation, genotoxic stress, DNA damage, oxidative stress, etc. The IkBα/NF-κB complex is tightly regulated by both phosphorylation and ubiquitination. Phosphorylation and ubiquitination of IκBα leads to its degradation and subsequent release of phosphorylated NF-κB to the nucleus. Greater than 100 target genes, including cytokines, cyclins, p21, Bcl-2, and c-Myc are known to be transcribed by NF-kB.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Noteso This is an overnight (4°C) incubation assay.
o This kit must be run using the provided Assay Buffer.
o This assay requires 25 μL diluted filtered cell lysate per well.
o The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • c-Myc
  • FADD (Ser194)
  • IκBα (Ser32)
  • IKKα/β (Ser177/Ser181)
  • NFκB (Ser536)
  • TNFR1
Assay PanelPremixed beads for your convenience.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
48-630MAG 04053252918766

Documentation

Protocols

Title
Protocol- 48-630MAG

MILLIPLEXMAP NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Brochure

Titre
Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling
The Power of Biomarker Analysis

Informations techniques

Titre
96 well Template 2 Up
96 well Template Single

Posters

Titre
Analysis of NF-kB Signaling Using Luminex®xMAP®Technology
MILLIPLEX®MAP Cell Signaling Wall Chart