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71086 KOD Hot Start DNA Polymerase

High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications.

KOD Hot Start DNA Polymerase : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
71086
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Products

RéférenceConditionnement Qté
71086-3 Boîte carton 200 u
71086-4 Flacon en verre 1000 u
71086-5 Ampoule plast. 20 u
Description
OverviewPCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore's molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility. KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'→5' exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start amplifies genomic DNA templates up to 21 kb including GC-rich genes for PCR applications. KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can occur during setup and initial temperature increase are avoided. In addition, primer degradation during setup at room temperature due to exonuclease activity is effectively inhibited. KOD Hot Start DNA Polymerase generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.

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Source Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli
Concentration 1.0 U/µl
Nicking activity None detected
Amplification effiency Functional PCR; inhibition of activity at 21°C verified
Storage –20°C

*Manufactured by Toyobo and distributed by EMD. Not available in Japan.

Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

Catalogue Number71086 Brand Family Novagen®
Features and benefits
  • Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
  • Amplifies genomic DNA templates up to 12 kbp
  • Amplifies plasmid and lambda DNA templates up to 21 kbp
  • Successfully amplifies GC-rich sequences
  • Eliminates mispriming and primer-dimer formation
  • Convenient room-temperature setup compatible with automation
  • Optimal KOD Hot Start Buffer for PCR performance over a wide range of targets
References
ReferencesMizuguchi, H., et al. 1999. J. Biochem. (Tokyo) 126, 762. Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [<Sup>3</Sup>H]dTTP), and 150 µg/ml activated calf thymus DNA.
Components
DeclarationManufactured by Toyobo and distributed by EMD. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
71086-3 07790788053017
71086-4 07790788060268
71086-5 04055977271720

Documentation

KOD Hot Start DNA Polymerase Certificats d'analyse

TitreNuméro de lot
71086

Références bibliographiques

Aperçu de la référence bibliographique
Mizuguchi, H., et al. 1999. J. Biochem. (Tokyo) 126, 762. Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.

Brochure

Titre
High fidelity gene amplification
PCR Protocols and Guides - Simplify your gene discovery
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

Citations

Titre
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  • Motoko Unoki, et al. (2006) Novel splice variants of ING4 and their possible roles in regulation of cell growth and motility. Journal of Biological Chemistry 281, 34677-34686.
  • Brock F. Binkowski, et al. (2005) Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Research 33, e66.
  • Sean Crosson, et al. (2005) Conserved modular design of an oxygen sensory/signaling network with species-specific output. Proceedings of the National Academy of Sciences (USA) 102, 8018-8023.
  • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
  • Oliver Schilling, et al. (2005) Exosite modules guide substrate recognition in the ZiPD/ElaC protein family. Journal of Biological Chemistry 280, 17857-17862.
  • Marko Tammenkoski, et al. (2005) An unusual, his-dependent family I pyrophosphatase from Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 41819-41826.
  • Mark E. Williams, et al. (2005) Ric-3 promotes functional expression of the nicotinic acetylcholine receptor 7 subunit in mammalian cells. Journal of Biological Chemistry 280, 1257-1263.
  • Jean-Francois Rual, et al. (2004) Human ORFeome version 1.1: a platform for reverse proteomics. Genome Research 14, 2128-2135.
  • Xinxin Gao, et al. (2003) Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences. Nucleic Acids Research 31, e143-.
  • Tomoko Miyazato, et al. (2003) Molecular analysis of VcfQ protein involved in Vibrio cholerae type IV pilus biogenesis. Medical Microbiology 52, 283-288.
  • Takaaki Sato, et al. (2003) Targeted gene disruption by homologous recombination in the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. Journal of Bacteriology 185, 210-220.
  • Yoshihiko Hirohashi, et al. (2002) An HLA-A24-restricted cytotoxic T lymphocyte epitope of a tumor-associated protein, survivin. Clinical Cancer Research 8, 1731-1739.
  • Takeshi Okamoto, et al. (2002) A change in PBP1 is involved in amoxicillin resistance of clinical isolates of Helicobacter pylori. Journal of Antimicrobial Chemotherapy 50, 849-856.
  • Mitsuaki Tabuchi, et al. (2002) Alternative splicing regulates the subcellular localization of divalent metal transporter 1 isoforms. Molecular Biology of the Cell 13, 4371-4387.
  • Harumi Terasaki, et al. (2002) Frizzled-10, up-regulated in primary colorectal cancer, is a positive regulator of the WNT-β-catenin-TCF signaling pathway. International Journal of Molecular Medicine 9, 107-112.
  • Masaki Watanabe, et al. (2002) Molecular characterization of a novel β1,3-galactosyltransferase for capsular polysaccharide synthesis by Streptococcus agalactiae type Ib. 131, 183-191.
  • Tokinori Iwamotob, et al. (2001) Establishment of an infectious RNA transcription system for striped jack nervous necrosis virus, the type species of the betanodaviruses. Journal of General Virology 82, 2653-2662.
  • Naoki Matsumoto, et al. (2001) H-2 allele specificity of the NK cell C-type lectin-like MHC class I receptor Ly49A visualized by soluble Ly49A tetramer. International Immunology 13, 615-623.
  • Naoki Matsumotoa, et al. (2001) The functional binding site for the C-type lectin-like natural killer cell receptor Ly49A spans three domains of its major histocompatibility complex class I ligand. Journal of Experimental Medicine 193, 147-158.
  • Norihiko Sagara and Masaru Katoh. (2000) Mitomycin C resistance induced by TCF-3 overexpression in gastric cancer cell line MKN28 is associated with DT-diaphorase down-regulation. Cancer Research 60, 5959-5962.
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    Protocoles Utilisateur

    Titre
    TB341 KOD Hot Start DNA Polymerase