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407652 Interleukin-6, Human, Recombinant, E. coli

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: rhIL-6

407652
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
407652-10UG Ampoule plast. 10 μg
Description
OverviewRecombinant, human, interleukin-6 expressed in E. coli. A prototypic pleiotrophic, pro-inflammatory cytokine that stimulates the production of acute phase proteins by hepatocytes. Also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B cells, T cells, and hepatocytes. Stimulates growth and inhibits constitutive, protein synthesis-independent apoptosis of murine B cell hybridoma 7TD1. IL-6 is produced by macrophages, endothelial cells, and activated T helper cells. Also considered as a potential marker of adipocyte differentiation. Human IL-6 is active on murine cells.
Catalogue Number407652
Brand Family Calbiochem®
SynonymsrhIL-6
References
ReferencesVicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord. 26, 9.
Vallieres, L., and Rivest, S. 1999. Endocrinology 140, 3890.
Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182.
Liu, J., et al. 1994. Cell Immunol. 155, 229.
Van Snick, J. 1990. Annu. Rev. Immunol. 8, 253.
Product Information
FormLyophilized
FormulationCarrier free.
Quality LevelMQ100
Applications
Biological Information
Biological activity8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg IL-6
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
407652-10UG 04055977211405

Documentation

Interleukin-6, Human, Recombinant, E. coli FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Interleukin-6, Human, Recombinant, E. coli Certificats d'analyse

TitreNuméro de lot
407652

Références bibliographiques

Aperçu de la référence bibliographique
Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord. 26, 9.
Vallieres, L., and Rivest, S. 1999. Endocrinology 140, 3890.
Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182.
Liu, J., et al. 1994. Cell Immunol. 155, 229.
Van Snick, J. 1990. Annu. Rev. Immunol. 8, 253.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-October-2007 JSW
SynonymsrhIL-6
DescriptionRecombinant, human, interleukin-6 expressed in E. coli. Interleukin-6 is a protein produced by a number of cell types including T-lymphocytes, monocytes, diploid fibroblasts, hepatocytes, cardiac myxomas, certain cervical cancer cells, bladder cell carcinomas, vascular endothelial cells, and human myelomas. It has a variety of biological effects and does not show a high degree of species specificity. Stimulates the production of acute phase proteins by hepatocytes. Also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B-cells, T-cells, and hepatocytes. Stimulates growth and inhibits constitutive protein synthesis-independent apoptosis of murine B-cell hybridoma 7TD1. Human sequence IL-6 is active on murine cells. 1 µg is equivalent to 7.0 x 103 WHO units.
FormLyophilized
FormulationCarrier free.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg IL-6
Biological activity8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.
SolubilityReconstitute to 0.1-1.0 mg/ml in 100 mM acetic acid.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesVicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord. 26, 9.
Vallieres, L., and Rivest, S. 1999. Endocrinology 140, 3890.
Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182.
Liu, J., et al. 1994. Cell Immunol. 155, 229.
Van Snick, J. 1990. Annu. Rev. Immunol. 8, 253.