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616464 InSolution™ TGF-β RI Kinase Inhibitor VI, SB431542 - CAS 301836-41-9 - Calbiochem

InSolution™ TGF-β RI Kinase Inhibitor VI, SB431542, CAS 301836-41-9, is a 100 mM solution in DMSO.A cell-permeable, selective inhibitor of SMAD2 phosphorylation (greater than 90% inhibition at 10 µM).

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
616464
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Aperçu

Replacement Information

Tableau de caractéristiques principal

CAS #Empirical Formula
301836-41-9C₂₂H₁₆N₄O₃ • 2H₂O

Products

RéférenceConditionnement Qté
616464-5MG Flacon en verre 5 mg
Description
OverviewA cell-permeable triarylimidazole compound that is shown to effectively inhibit cellular Smad2 phosphorylation (>90% inhibition by 10 µM inhibitor) upon vector-mediated expression of constitutively active ALK4, ALK5, or ALK7 in NIH 3T3 cells, while exhibiting little effect against Smad1 phosphorylation by other members of type I receptors for TGF-β in NIH 3T3 cultures expressing active ALK1, 2, 3, or 6. When tested directly in cell-free kinase assays, SB431542 is demonstrated to potently inhibit the activity of ALK4 and ALK5 (IC50 = 140 nM and 94 nM, respectively) with no or much reduced potency toward a panel of 24 other kinases (IC50 ≥10 µM in the presence of 10 µM ATP), including ALK2 and ALK6. Reported to improve the efficiency of 4-TF-induced human iPSCs generation from fibroblast cultures by >200-fold when used together with PD0325901 (Cat. No. 444966) and Thiazovivin (Cat. No. 420220). The solid form of this compound (Cat. No. 616461) is also available.
Catalogue Number616464
Brand Family Calbiochem®
References
ReferencesIkushima, H., et al. 2009. Cell Stem Cell 5, 504.
Lin, T., et al. 2009. Nat. Methods 6, 805.
Maherali, N. and Hochedlinger, K., 2009. Curr. Biol. 19, 1718.
Callahan, J.F., et al. 2002. J. Med. Chem. 45, 999.
Inman, G.J., et al. 2002. Mol. Pharmacol. 62, 65.
Laping, N.J., et al. 2002. Mol. Pharmacol. 62, 58.
Product Information
CAS number301836-41-9
FormLiquid
FormulationA 100 mM (5 mg/119 µl) solution of SB431542 (Cat. No. 616461) in DMSO.
Hill FormulaC₂₂H₁₆N₄O₃ • 2H₂O
Chemical formulaC₂₂H₁₆N₄O₃ • 2H₂O
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationInSolution™ TGF-β RI Kinase Inhibitor VI, SB431542, CAS 301836-41-9, is a 100 mM solution in DMSO.A cell-permeable, selective inhibitor of SMAD2 phosphorylation (greater than 90% inhibition at 10 µM).
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
616464-5MG 04055977185942

Documentation

InSolution™ TGF-β RI Kinase Inhibitor VI, SB431542 - CAS 301836-41-9 - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

InSolution™ TGF-β RI Kinase Inhibitor VI, SB431542 - CAS 301836-41-9 - Calbiochem Certificats d'analyse

TitreNuméro de lot
616464

Références bibliographiques

Aperçu de la référence bibliographique
Ikushima, H., et al. 2009. Cell Stem Cell 5, 504.
Lin, T., et al. 2009. Nat. Methods 6, 805.
Maherali, N. and Hochedlinger, K., 2009. Curr. Biol. 19, 1718.
Callahan, J.F., et al. 2002. J. Med. Chem. 45, 999.
Inman, G.J., et al. 2002. Mol. Pharmacol. 62, 65.
Laping, N.J., et al. 2002. Mol. Pharmacol. 62, 58.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-February-2012 RFH
DescriptionA cell-permeable triarylimidazole compound that is shown to effectively inhibit cellular Smad2 phosphorylation (>90% inhibition by 10 µM inhibitor) upon vector-mediated expression of constitutively active ALK4, ALK5, or ALK7 in NIH 3T3 cells, while exhibiting little effect against Smad1 phosphorylation by other members of type I receptors for TGF-β in NIH 3T3 cultures expressing active ALK1, 2, 3, or 6. When tested directly in cell-free kinase assays, SB431542 is demonstrated to potently inhibit the activity of ALK4 and ALK5 (IC50 = 140 nM and 94 nM, respectively) with no or much reduced potency toward a panel of 24 other kinases (IC50 ≥10 µM in the presence of 10 µM ATP), including ALK2 and ALK6. Reported to improve the efficiency of 4-TF-induced human iPSCs generation from fibroblast cultures by >200-fold when used together with PD0325901 (Cat. No. 444966) and Thiazovivin (Cat. No. 420220).
FormLiquid
FormulationA 100 mM (5 mg/119 µl) solution of SB431542 (Cat. No. 616461) in DMSO.
Intert gas (Yes/No) Packaged under inert gas
CAS number301836-41-9
Chemical formulaC₂₂H₁₆N₄O₃ • 2H₂O
Structure formulaStructure formula
Purity≥97% by HPLC
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesIkushima, H., et al. 2009. Cell Stem Cell 5, 504.
Lin, T., et al. 2009. Nat. Methods 6, 805.
Maherali, N. and Hochedlinger, K., 2009. Curr. Biol. 19, 1718.
Callahan, J.F., et al. 2002. J. Med. Chem. 45, 999.
Inman, G.J., et al. 2002. Mol. Pharmacol. 62, 65.
Laping, N.J., et al. 2002. Mol. Pharmacol. 62, 58.