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559398 InSolution™ SB 203580 - Calbiochem

InSolution SB 203580, CAS 152121-47-6, is a 1 mg/ml solution of SB 203580 in DMSO. A cell-permeable, selective, reversible inhibitor of p38 MAP kinase (IC₅₀ = 34 nM in vitro, 600 nM in cells).

InSolution™ SB 203580 - Calbiochem : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: InSolution™ p38 MAP Kinase Inhibitor XVI

Primary Target: P38MAPK
559398
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Empirical Formula
C₂₁H₁₆FN₃OS

Products

RéférenceConditionnement Qté
559398-1ML Ampoule plast. 1 ml
Description
Catalogue Number559398
Brand Family Calbiochem®
SynonymsInSolution™ p38 MAP Kinase Inhibitor XVI
References
ReferencesPowell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
Davies, S.P., et al. 2000. Biochem. J. 351, 95.
Iwasaki, S., et al. 1999. J. Biol. Chem. 274, 26503.
Gallagher, T.F., et al. 1997. Bioorg. Med. Chem. 5, 49.
LoGrasso, P.V., et al. 1997. Biochem. 36, 10422.
Hazzalin, C.A., et al. 1996. Curr. Biol. 6, 1028.
Kramer, R.M., et al. 1996. J. Biol. Chem. 271, 27723.
Saklatvala, J., et al. 1996. J. Biol. Chem. 271, 6586.
Cuenda, A., et al. 1995. FEBS Lett. 364, 229.
Gallagher, T.F., et al. 1995. Bioorg. Med. Chem. Lett. 5, 1171.
Lee, J.C., et al. 1994. Nature 372, 739.
Product Information
ATP CompetitiveY
FormLiquid
FormulationA 1 mg/ml solution of SB 203580 (Cat. No. 559389) in anhydrous DMSO.
Hill FormulaC₂₁H₁₆FN₃OS
Chemical formulaC₂₁H₁₆FN₃OS
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationInSolution SB 203580, CAS 152121-47-6, is a 1 mg/ml solution of SB 203580 in DMSO. A cell-permeable, selective, reversible inhibitor of p38 MAP kinase (IC₅₀ = 34 nM in vitro, 600 nM in cells).
Biological Information
Primary TargetP38MAPK
Primary Target IC<sub>50</sub>34 nM in vitro, 600 nM in cells against p38 MAPK; 50-100 nM Inhibiting IL-1 and TNF-α production from LPS-stimulated human monocytes and the human monocyte cell line THP-1; 0.2-1.0 µM inhibiting platelet aggregation by collagen
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 24/25-26

Avoid contact with skin and eyes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot, purge with inert gas, and freeze (-20°C).
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
559398-1ML 04055977192636

Documentation

InSolution™ SB 203580 - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

InSolution™ SB 203580 - Calbiochem Certificats d'analyse

TitreNuméro de lot
559398

Références bibliographiques

Aperçu de la référence bibliographique
Powell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
Davies, S.P., et al. 2000. Biochem. J. 351, 95.
Iwasaki, S., et al. 1999. J. Biol. Chem. 274, 26503.
Gallagher, T.F., et al. 1997. Bioorg. Med. Chem. 5, 49.
LoGrasso, P.V., et al. 1997. Biochem. 36, 10422.
Hazzalin, C.A., et al. 1996. Curr. Biol. 6, 1028.
Kramer, R.M., et al. 1996. J. Biol. Chem. 271, 27723.
Saklatvala, J., et al. 1996. J. Biol. Chem. 271, 6586.
Cuenda, A., et al. 1995. FEBS Lett. 364, 229.
Gallagher, T.F., et al. 1995. Bioorg. Med. Chem. Lett. 5, 1171.
Lee, J.C., et al. 1994. Nature 372, 739.

Citations

Titre
  • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
  • Bysani Chandrasekar, et al. (2005) The pro-atherogenic cytokine interleukin-18 induces CXCL16 expression in rat aortic smooth muscle cells via MyD88, interleukin-1 receptor-associated kinase, tumor necrosis factor receptor-associated factor 6, c-Src, phosphatidylinositol 3-kinase, Akt, c-J. Journal of Biological Chemistry 280, 26263-26277.
  • Guadalupe Sabio, et al. (2005) p38γ regulates the localisation of SAP97 in the cytoskeleton by modulating its interaction with GKAP. The EMBO Journal 24, 1134-1145.
  • Jose G. Trevino, et al. (2005) Expression and activity of Src regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Research 65, 7214-7222.
  • Wojciech Wojciechowski, et al. (2005) Enhanced Expression of CD20 in Human Tumor B Cells is Controlled Through ERK-Dependent Mechanisms. Journal of Immunology 174, 7859-7868.
  • Yung-Luen Yu, et al. (2005) MAP kinase-mediated phosphorylation of GATA-1 promotes Bcl-XL expression and cell survival. Journal of Biological Chemistry 280, 29533-29542.
  • Naoto Inukai, et al. (2004) A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. Journal of Biological Chemistry 279, 8190-8195.
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    Fiche technique

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision05-October-2007 RFH
    SynonymsInSolution™ p38 MAP Kinase Inhibitor XVI
    DescriptionA highly specific, cell-permeable inhibitor of p38 kinase (IC50 = 34 nM in vitro, 600 nM in cells). Also known as reactivating kinase (RK) and CSBP (cytokine synthesis anti-inflammatory drug binding protein). Does not significantly inhibit JNK or p42 MAP kinase even at 100 µM. Inhibits IL-1 and TNF-α production from LPS-stimulated human monocytes and the human monocyte cell line THP-1 (IC50 = 50-100 nM). SB 203580 has also been shown to be an effective inhibitor of inflammatory cytokine production in vivo in both mice and rats.
    FormLiquid
    FormulationA 1 mg/ml solution of SB 203580 (Cat. No. 559389) in anhydrous DMSO.
    Intert gas (Yes/No) Packaged under inert gas
    Chemical formulaC₂₁H₁₆FN₃OS
    Structure formulaStructure formula
    Purity≥98% by HPLC
    Storage Protect from light
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot, purge with inert gas, and freeze (-20°C).
    Toxicity Irritant
    ReferencesPowell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
    Davies, S.P., et al. 2000. Biochem. J. 351, 95.
    Iwasaki, S., et al. 1999. J. Biol. Chem. 274, 26503.
    Gallagher, T.F., et al. 1997. Bioorg. Med. Chem. 5, 49.
    LoGrasso, P.V., et al. 1997. Biochem. 36, 10422.
    Hazzalin, C.A., et al. 1996. Curr. Biol. 6, 1028.
    Kramer, R.M., et al. 1996. J. Biol. Chem. 271, 27723.
    Saklatvala, J., et al. 1996. J. Biol. Chem. 271, 6586.
    Cuenda, A., et al. 1995. FEBS Lett. 364, 229.
    Gallagher, T.F., et al. 1995. Bioorg. Med. Chem. Lett. 5, 1171.
    Lee, J.C., et al. 1994. Nature 372, 739.
    Citation
  • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
  • Bysani Chandrasekar, et al. (2005) The pro-atherogenic cytokine interleukin-18 induces CXCL16 expression in rat aortic smooth muscle cells via MyD88, interleukin-1 receptor-associated kinase, tumor necrosis factor receptor-associated factor 6, c-Src, phosphatidylinositol 3-kinase, Akt, c-J. Journal of Biological Chemistry 280, 26263-26277.
  • Guadalupe Sabio, et al. (2005) p38γ regulates the localisation of SAP97 in the cytoskeleton by modulating its interaction with GKAP. The EMBO Journal 24, 1134-1145.
  • Jose G. Trevino, et al. (2005) Expression and activity of Src regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Research 65, 7214-7222.
  • Wojciech Wojciechowski, et al. (2005) Enhanced Expression of CD20 in Human Tumor B Cells is Controlled Through ERK-Dependent Mechanisms. Journal of Immunology 174, 7859-7868.
  • Yung-Luen Yu, et al. (2005) MAP kinase-mediated phosphorylation of GATA-1 promotes Bcl-XL expression and cell survival. Journal of Biological Chemistry 280, 29533-29542.
  • Naoto Inukai, et al. (2004) A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. Journal of Biological Chemistry 279, 8190-8195.
    		•