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505224 InSolution™ Necrostatin-1 - Calbiochem

InSolution™ Necrostatin-1 - Calbiochem : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Necrosis Inhibitor II, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protein 1 Inhibitor I

505224
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Empirical Formula
C₁₃H₁₃N₃OS

Products

RéférenceConditionnement Qté
5052240001 Flacon en verre 10 mg
Description
OverviewA cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect on RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (Cat. No. 480066).
Catalogue Number505224
Brand Family Calbiochem®
SynonymsNecrosis Inhibitor II, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protein 1 Inhibitor I
References
ReferencesDegterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
Muller, A.J., et al. 2005. Nat. Med. 11, 312.
Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.
Product Information
FormLiquid
FormulationA 25 mM (10 mg/1.54 ml) sterile-filtered solution of Necrostatin-1 (Cat. No. 480065) in DMSO.
Hill FormulaC₁₃H₁₃N₃OS
Chemical formulaC₁₃H₁₃N₃OS
Hygroscopic Hygroscopic
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥99% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 26-36-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
5052240001 04055977263473

Documentation

InSolution™ Necrostatin-1 - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Références bibliographiques

Aperçu de la référence bibliographique
Degterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
Muller, A.J., et al. 2005. Nat. Med. 11, 312.
Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-December-2013 JSW
SynonymsNecrosis Inhibitor II, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protein 1 Inhibitor I
DescriptionA cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect on RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (Cat. No. 480066).
FormLiquid
FormulationA 25 mM (10 mg/1.54 ml) sterile-filtered solution of Necrostatin-1 (Cat. No. 480065) in DMSO.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₃H₁₃N₃OS
Structure formulaStructure formula
Purity≥99% by HPLC
Storage Protect from light
Avoid freeze/thaw
≤ -70°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.
Toxicity Irritant
ReferencesDegterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
Muller, A.J., et al. 2005. Nat. Med. 11, 312.
Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.