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IPFL20200 Immobilon-FL en PVDF, 0,45 µm, feuille de 20 x 20 cm

Western blotting, dot/slot

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Principales applications: Western Blotting, Dot Blot
IPFL20200
10  
Purchase on Sigma-Aldrich

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Replacement Information

Tableau de caractéristiques principal

Principales applications
WB, DB
Description
RéférenceIPFL20200
Nom de marque
  • Immobilon
DescriptionImmobilon-FL en PVDF, 0,45 µm, feuille de 20 x 20 cm
Informations généralesOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
Références bibliographiques
Informations produit
Méthode de détectionFluorescence Chimifluorescence Chromogénie Chimiluminescence
Code SH3921 19 00
Colorants compatibles
  • Bleu brillant de Coomassie
  • Amido black
  • Rouge Ponceau-S
  • CPTS
Code du filtreIPFL
Couleur du filtreBlanc
Quality LevelMQ400
Applications
ApplicationWestern blotting, dot/slot
Principales applications
  • Western Blotting
  • Dot Blot
Informations biologiques
Adsorption de BSA205 µg/cm²
Adsorption d'IgG de chèvre300 µg/cm²
Adsorption d'insuline155 µg/cm²
MouillabilitéHydrophobe
Informations physico-chimiques
Dimension de pores0.45 µm
Dimensions
Surface du filtreUnie
Dimensions du filtre20 cm x 20 cm
Informations sur les matériaux
Chimie
  • PVDF hydrophobe
Informations toxicologiques
Informations de sécurité selon le SGH
Informations sur la sécurité
Notifications sur l'utilisation du produit
Informations relatives au stockage et à l'expédition
Informations sur l'emballage
Quantité10
Informations sur le transport
Information complémentaire
Caractéristiques
Global Trade Item Number
Référence GTIN
IPFL20200 04053252671920