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IHCR2025-6 IHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3

IHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 is an antibody against Cytokeratin AE1/AE3 (Pan cytokeratins) for use in IH(P).

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Cytokeratins
IHCR2025-6
6 mL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberIHCR2025-6
Trade Name
  • IHC Select
  • Chemicon
DescriptionIHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3
OverviewKeratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament. These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis (Moll, R., 1982). These can be divided into acid (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56, and 52 kD. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state (Sun, T.T., 1984; Cooper, D., 1985; Sun, T. T., 1985 ). The 56.5/65-67 kD pair is present in keratinized (differentiated) epidermis. The 55/64 kD pair is characteristic of normal (corneal-type) epithelial differentiation (Moll, R., 1982; Sun, T.T, 1984). The 51/59 kD pair is characteristic of the stratified squamous epithelial of internal organism such as esophagus and tongue (Moll, R., 1982; Cooper, D., 1985). The 51/58 kD pair is a keratinocyte marker; this pair is present in almost all stratified epithelia irrespective of the state of cellular stratification (Moll, R., 1982; Sun, T.T., 1984). The 48/56 kD pair is characteristic of hyper-proliferative (de-differentiated) keratinocytes (Moll, R., 1982; Weiss, R.A., 1984). The 45/52 kD pair and to a lesser extent, the 46/54 kD pair are characteristic of simple epithelia (Moll, R., 1982). The 40 kD keratin is present in most epithelia except adult epidermis (Moll, R., 1982).
References
Product Information
FormatPurified
PresentationPurified monoclonal mouse antibody supplied in liquid format diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.
Quality LevelMQ100
Applications
ApplicationIHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 is an antibody against Cytokeratin AE1/AE3 (Pan cytokeratins) for use in IH(P).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesAntibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 10 minutes with IHC Select® Detection Kits.

Cytokeratin AE1/AE3 has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2025-6.
Biological Information
ImmunogenHuman epidermal keratin
CloneAE1/AE3
HostMouse
SpecificityAnti-Keratin AE1 recognizes the 56.5, 50, 50', 48, and 40 kD keratins of the acidic subfamily. Anti-keratin AE3 recognizes all members of the basic subfamily. AE1/AE3 reacts with both acidic and basic keratins. Staining is localized to the cytoplasm. AE1/AE3 specifically binds to antigens located in the cytoplasm of normal epithelia. Reacts with cells of epithelial origin including simple and stratified epithelia and epidermis.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C . Refer to vial for expiration dating.
Packaging Information
Material Size6 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
IHCR2025-6 04053252627804

Documentation

IHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

IHC Select® Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 Certificats d'analyse

TitreNuméro de lot
MILLIPORE IHC SELECT RESEARCH PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL - 3227854 3227854
MILLIPORE IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY - 1977809 1977809
MILLIPORE IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY - 2001344 2001344
MILLIPORE IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY - 2059294 2059294
MILLIPORE IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY - 2192722 2192722
MILLIPORE IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL - 3071900 3071900
MILLIPORE IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL - 3160117 3160117
MILLIPORE IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY 2909552
MILLIPORE IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-HUMAN CYTOKERATIN EPITHELIAL MONOCLONAL ANTIBODY 3008184
MILLIPORE IHC SELECT RESEARCH -2800503 2800503
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Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
Galanin modulates the neural niche to favour perineural invasion in head and neck cancer.
Scanlon, CS; Banerjee, R; Inglehart, RC; Liu, M; Russo, N; Hariharan, A; van Tubergen, EA; Corson, SL; Asangani, IA; Mistretta, CM; Chinnaiyan, AM; D'Silva, NJ
Nat Commun  6  6885  2015

Afficher le résumé 		25917569 25917569

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Cytokeratin Antibodies

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Produits associés classés par : Brand Facete

IHC Select®

Catégories

Life Science Research > Antibodies and Assays > Primary Antibodies