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407255 IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant

407255
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Purity
≥97% by SDS-PAGE

Products

RéférenceConditionnement Qté
407255-25UG Flacon en verre 25 μg
Description
OverviewRecombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag®. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.
Catalogue Number407255
Brand Family Calbiochem®
SynonymsIGFBP-7
References
ReferencesAhmed, S. et al. 2003. Biochem. Biophys. Res. Commun. 310, 612.
Nagakubo, D. et al. 2003. J. Immunol. 171, 553.
Wilson, HM. et al. 2002. Cell Growth Differ. 13, 205.
Degeorges, A. et al. 2000. J. Histochem. Cytochem. 48, 747.
Kato, M.V. 2000. Mol. Med. 6, 126.
Hwa, V. et al. 1999. Endocr. Rev. 20, 761.
Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA 94,12981.
Kato, M.V. et al. 1996. Oncogene 12, 1361.
Oh, Y. et al. 1996. J. Biol. Chem. 271, 30322.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered PBS.
Quality LevelMQ100
Applications
Biological Information
Biological activityFunctional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU per/1 µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
407255-25UG 04055977188738

Documentation

IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant Certificats d'analyse

TitreNuméro de lot
407255

Références bibliographiques

Aperçu de la référence bibliographique
Ahmed, S. et al. 2003. Biochem. Biophys. Res. Commun. 310, 612.
Nagakubo, D. et al. 2003. J. Immunol. 171, 553.
Wilson, HM. et al. 2002. Cell Growth Differ. 13, 205.
Degeorges, A. et al. 2000. J. Histochem. Cytochem. 48, 747.
Kato, M.V. 2000. Mol. Med. 6, 126.
Hwa, V. et al. 1999. Endocr. Rev. 20, 761.
Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA 94,12981.
Kato, M.V. et al. 1996. Oncogene 12, 1361.
Oh, Y. et al. 1996. J. Biol. Chem. 271, 30322.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-Sepmtember-2011 RFH
SynonymsIGFBP-7
DescriptionRecombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag®. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered PBS.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU per/1 µg protein
Biological activityFunctional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.
SolubilityReconstitute to 100 µg/ml in sterile PBS.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesAhmed, S. et al. 2003. Biochem. Biophys. Res. Commun. 310, 612.
Nagakubo, D. et al. 2003. J. Immunol. 171, 553.
Wilson, HM. et al. 2002. Cell Growth Differ. 13, 205.
Degeorges, A. et al. 2000. J. Histochem. Cytochem. 48, 747.
Kato, M.V. 2000. Mol. Med. 6, 126.
Hwa, V. et al. 1999. Endocr. Rev. 20, 761.
Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA 94,12981.
Kato, M.V. et al. 1996. Oncogene 12, 1361.
Oh, Y. et al. 1996. J. Biol. Chem. 271, 30322.