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SCM110 Human ES/iPS Neural Induction Medium

SCM110
125ml/Kit  
Purchase on Sigma-Aldrich

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Key Applications
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Description
Catalogue NumberSCM110
DescriptionHuman ES/iPS Neural Induction Medium
Alternate Names
  • NIM
Background InformationEMD Millipore’s Human ES/iPS Neural Induction Mediium is an serum free/cheimcally defined optimized media for the rapid production of expandable neural progenitor cells from pluripotent human ES and iPS cells. The media relies on both small molecules neural inducers and supplements to generate a highly enriched population of expandable neural progenitors based on establish protocols. Neural progenitors are generated 10 days from starting cultures of traditional feeder-based and/or feeder-free cultures of undifferentiated human ES/iPS cells and can be expanded for over 3-5 passages resulting in a minimum 20-fold expansion. Typically, from 1 well of a 6-well plate containing an estimated 500,000 pluripotent human ES/iPS, approximately 5-10 million neural progenitors can be obtained after 3 passages. The resulting neural progenitor cells are multipotent and can be further differentiated to specific neural cell subtypes of interest.
References
Product Information
Components
  • 1) Neural Induction Basal Medium (Part No. CS210991). One 125 mL bottle.
  • 2) Neural Supplement 1 (50X) (Part No. CS210992). One 2.5 mL bottle.
  • 3) Neuro2 Medium Supplement (Part No. SCM012-S). One 1.25 mL bottle.
  • 4) GSK3 and TGFbR Inhibitor Cocktail (1000X) (Part No. CS210983-150UL). One 150 µL vial.
  • 5) AMPK Inhibitor (1000X) (Part No. CS210995-60UL). One 60 µL vial.
Quality LevelMQ100
Applications
ApplicationEMD Millipore’s Human ES/iPS Neural Induction Mediium is an serum free/chemically defined optimized media for the rapid production of expandable neural progenitor cells from pluripotent human ES and iPS cells. 
Key Applications
  • Cell Differentiation
Biological Information
Media FormLiquid
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20C. Good for 3 months from date of receipt or until expiration date on bottle when reagents are handled and stored appropriately. Please note: The supplemented ES/iPS Neural Induction Medium should be stored at 2 to 8C for up to 4 weeks.
Packaging Information
Material Size125ml/Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
SCM110 04053252855108

Documentation

Human ES/iPS Neural Induction Medium FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Brochure

Titre
Simplifying the iPSC Workflow

Informations techniques

Titre
Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

Posters

Titre
A unifying neural differentiation platform to direct lineage-specific neurons and glial cells from human pluripotent stem cells

Manuels d'utilisation

Titre
Human ES/iPS Neurogenesis Kit
User Guide - SCM110