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345749 GDF-8 Propeptide, His•Tag®, Mouse, Recombinant

GDF-8 Propeptide, His•Tag®, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Myostatin Propeptide

345749
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Purity
≥90% by SDS-PAGE

Products

RéférenceConditionnement Qté
345749-25UG Flacon en verre 25 μg
Description
OverviewRecombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.
Catalogue Number345749
Brand Family Calbiochem®
SynonymsMyostatin Propeptide
References
ReferencesHill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
Thies, R.S. et al. 2001. Growth Factors 18, 251.
Chang, H. et al. 2002. Endocrine Reviews 23, 787.
McPherron, A.C. et al. 1997. Nature 387, 83.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Quality LevelMQ100
Applications
ApplicationGDF-8 Propeptide, His•Tag®, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells.
Biological Information
Biological activityED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
Purity≥90% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
345749-25UG 04055977193893

Documentation

GDF-8 Propeptide, His•Tag®, Mouse, Recombinant FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

GDF-8 Propeptide, His•Tag®, Mouse, Recombinant Certificats d'analyse

TitreNuméro de lot
345749

Références bibliographiques

Aperçu de la référence bibliographique
Hill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
Thies, R.S. et al. 2001. Growth Factors 18, 251.
Chang, H. et al. 2002. Endocrine Reviews 23, 787.
McPherron, A.C. et al. 1997. Nature 387, 83.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-June-2011 RFH
SynonymsMyostatin Propeptide
DescriptionRecombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Purity≥90% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Biological activityED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
SolubilityReconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesHill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
Thies, R.S. et al. 2001. Growth Factors 18, 251.
Chang, H. et al. 2002. Endocrine Reviews 23, 787.
McPherron, A.C. et al. 1997. Nature 387, 83.