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14-720 FAK Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human FAK, amino acids 411-686, for use in Kinase Assays.

FAK Protein, active, 10 µg : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
14-720
10 µg  
Purchase on Sigma-Aldrich

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Replacement Information
Description
Catalogue Number14-720
Brand Family Upstate
Trade Name
  • Upstate
DescriptionFAK Protein, active, 10 µg
OverviewN-terminal 6His-tagged, recombinant, human FAK, amino acids 411-686
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human FAK, amino acids 411-686, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
Gene Symbol
  • PTK2
  • pp125FAK
  • FAK
  • FADK
  • FAK1
Protein TargetFAK
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
Molecular Weight35.5kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of 100μM substrate peptide (EEEEYEEEEEEYYIIEEEEEEYEEEEEEYYEEEEEEKKK)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
14-720 04053252584510

Produits & Applications associés

Alternative Packsize

Référence Description
14-720M FAK Protein, active, 250 µg

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Catégories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases