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EPA102 EPA II Peroxidase Protection Buffer

500 mL EPA II Peroxidase Protection Buffer for ELISA & IHC.

EPA II Peroxidase Protection Buffer : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Key Applications: ELISA, Immunohistochemistry
EPA102
500 mL  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Key Applications
ELISA, IHC
Description
Catalogue NumberEPA102
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionEPA II Peroxidase Protection Buffer
OverviewEPA II Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods.
References
Product Information
PresentationEPA II Buffer contains 0.5% (w/v) bovine serum albumin, antimicrobial preservatives and stabilizing compounds in phosphate buffered saline, pH 7.2.
Applications
Application500 mL EPA II Peroxidase Protection Buffer for ELISA & IHC.
Key Applications
  • ELISA
  • Immunohistochemistry
Biological Information
Physicochemical Information
Dimensions
Volume500 mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2° to 8°C, for up to 6 months.

Chemicon HRP conjugated antibodies may be diluted up to 1:16,000 in EPA II Buffer. Diluted conjugates are stable for at least 3 months when stored at 2° to 8°C. The buffer is suitable for direct use in ELISA.

EPA II Buffer is compatible with blocking agents such as skim milk powder, Tween 20 and normal serum. However HRP conjugates should not be stored in the presence of these blocking agents. They should be added just prior to use in the assay.

PRECAUTIONS:

HRP conjugates should be diluted and stored under clean conditions to reduce the level of microbial challenge.

Sodium azide must NOT be added to EPA II Buffer.

Avoid contamination of HRP conjugate solutions with heavy metal ions, aromatic chlorocarbons or hypochlorous acids. Avoid containers made of soda glass or polystyrene. Storage of diluted conjugates in borosilicate glass is recommended.

Efficient washing steps should follow HRP conjugate incubations. In ELISA, at least four thorough wash cycles are recommended.
Packaging Information
Material Size500 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
EPA102 04053252326462

Documentation

EPA II Peroxidase Protection Buffer FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

EPA II Peroxidase Protection Buffer Certificats d'analyse

TitreNuméro de lot
PEROXIDASE PROTECTIVE BUFFER II 2902237
PEROXIDASE PROTECTIVE BUFFER II 2957054
PEROXIDASE PROTECTIVE BUFFER II 2865471
PEROXIDASE PROTECTIVE BUFFER II -
PEROXIDASE PROTECTIVE BUFFER II - 2401312 2401312
PEROXIDASE PROTECTIVE BUFFER II - 2324366 2324366
PEROXIDASE PROTECTIVE BUFFER II - 3251333 3251333
PEROXIDASE PROTECTIVE BUFFER II - 3674973 3674973
PEROXIDASE PROTECTIVE BUFFER II - 3832819 3832819
PEROXIDASE PROTECTIVE BUFFER II - 3930971 3930971
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Fiche technique

Titre
PEROXIDASE PROTECTIVE BUFFER II