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371207 Dkk-1, Mouse, Recombinant

Dkk-1, Mouse, Recombinant , consists of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

Dkk-1, Mouse, Recombinant : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
371207
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Purity
≥95% by SDS-PAGE

Products

RéférenceConditionnement Qté
371207-10UG Flacon en verre 10 μg
Description
OverviewRecombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND50 = 0.02-0.1 µg/ml.
Catalogue Number371207
Brand Family Calbiochem®
References
ReferencesPinzone, J.J., et al. 2009. Blood 113, 517.
Li, J., et al. 2006. Bone 36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C., 2006. Oncogene 25, 7469.
Verani, R., et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I., et al. 2005. J. Neurosci. 25, 2647.
Kemp, C., et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M., et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B., et al. 2002. Nature 417, 664.
Mao, B., et al. 2001. Nature 411, 321.
Glinka, A., et al. 1998. Nature 391, 357.
Product Information
FormLiquid
FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
371207-10UG 04055977191790

Documentation

Dkk-1, Mouse, Recombinant FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Dkk-1, Mouse, Recombinant Certificats d'analyse

TitreNuméro de lot
371207

Références bibliographiques

Aperçu de la référence bibliographique
Pinzone, J.J., et al. 2009. Blood 113, 517.
Li, J., et al. 2006. Bone 36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C., 2006. Oncogene 25, 7469.
Verani, R., et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I., et al. 2005. J. Neurosci. 25, 2647.
Kemp, C., et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M., et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B., et al. 2002. Nature 417, 664.
Mao, B., et al. 2001. Nature 411, 321.
Glinka, A., et al. 1998. Nature 391, 357.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-Sepmtember-2011 RFH
DescriptionRecombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND50 = 0.02-0.1 µg/ml.
FormLiquid
FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.
SolubilityReconstitute to 100 µg/ml in PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesPinzone, J.J., et al. 2009. Blood 113, 517.
Li, J., et al. 2006. Bone 36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res. 21, 934.
Niehrs, C., 2006. Oncogene 25, 7469.
Verani, R., et al. 2006. J. Neurochem. 101, 242.
Cappuccio, I., et al. 2005. J. Neurosci. 25, 2647.
Kemp, C., et al. 2005. Dev. Dyn. 233, 1064.
Adamska, M., et al. 2004. Dev. Biol. 272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B., et al. 2002. Nature 417, 664.
Mao, B., et al. 2001. Nature 411, 321.
Glinka, A., et al. 1998. Nature 391, 357.