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309800 Dihydroethidium - CAS 38483-26-0 - Calbiochem

309800
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Aperçu

Tableau de caractéristiques principal

CAS #Empirical Formula
38483-26-0C₂₁H₂₁N₃

Products

RéférenceConditionnement Qté
309800-25MG Flacon en verre 25 mg
Description
OverviewA cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
Catalogue Number309800
Brand Family Calbiochem®
Synonyms2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
References
ReferencesHaugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
Product Information
CAS number38483-26-0
ATP CompetitiveN
FormPink solid
Hill FormulaC₂₁H₂₁N₃
Chemical formulaC₂₁H₂₁N₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Biological Information
Primary TargetUseful fluorogenic probe for the detection of reactive oxygen species (ROS)
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Safety Information according to GHS
RTECSSF7935000
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Référence GTIN
309800-25MG 04055977216806