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260913 DNase I, Bovine Pancreas

Native DNase I from bovine pancreas.

DNase I, Bovine Pancreas : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Deoxyribonuclease I

260913
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
260913-10MU Flacon en verre 10 mu
260913-25MU Ampoule plast. 25 mu
Description
OverviewNative DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units
Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.
Catalogue Number260913
Brand Family Calbiochem®
SynonymsDeoxyribonuclease I
References
ReferencesTakeshita, H., et al. 2000. Biochem. Biophys. Res. Commun. 269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol. 264, 1154.
Product Information
CAS number9003-98-9
Activity≥60,000 Dornase units/mg dry weight.
Unit of DefinitionOne Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.
EC number3.1.21.1
FormLyophilized
Hygroscopic Hygroscopic
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSRF0750000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
260913-10MU 07790788049010
260913-25MU 04055977217506

Documentation

DNase I, Bovine Pancreas FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

DNase I, Bovine Pancreas Certificats d'analyse

TitreNuméro de lot
260913

Références bibliographiques

Aperçu de la référence bibliographique
Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun. 269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol. 264, 1154.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-April-2008 RFH
SynonymsDeoxyribonuclease I
DescriptionNative DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units
FormLyophilized
CAS number9003-98-9
RTECSRF0750000
EC number3.1.21.1
Activity≥60,000 Dornase units/mg dry weight.
Unit definitionOne Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.
SolubilitySodium Acetate Buffer or H₂O
Storage Protect from moisture
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
Merck USA index14, 2906
ReferencesTakeshita, H., et al. 2000. Biochem. Biophys. Res. Commun. 269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol. 264, 1154.