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14-703M Cot1 Protein, active, 250 µg

Active, N-terminal 6His-tagged recombinant human Cot1, amino acids 30-397. For use in Kinase Assays.

14-703M
250 µg  Also available in 10 μg size (cat#14-703) and in bulk (cat#14-703-K).
Purchase on Sigma-Aldrich

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Replacement Information
Description
Catalogue Number14-703M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCot1 Protein, active, 250 µg
OverviewN-terminal 6His-tagged recombinant human Cot1, amino acids 30-397.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal 6His-tagged recombinant human Cot1, amino acids 30-397. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman Cot1, amino acids 30-397, expressed by baculovirus in Sf21 insect cells.
Entrez Gene Number
Entrez Gene SummaryThis gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.
Gene Symbol
  • MAP3K8
  • TPL2
  • FLJ10486
  • C-COT
  • COT
  • c-COT
  • ESTF
  • EST
  • Tpl-2
Protein TargetCot1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P41279 # Able to activate NF-kappa-B 1 by stimulating proteasome- mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form of cot has some transforming activity, although it is much weaker than the activated cot oncoprotein.
SIZE: 467 amino acids; 52925 Da
SUBUNIT: Interacts with NFKB1/p105.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in several normal tissues and human tumor-derived cell lines.
DEVELOPMENTAL STAGE: The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle.
PTM: Autophosphorylated. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.
SIMILARITY: SwissProt: P41279 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight45.3kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by activation of MEK1 (14-420). The activated MEK1 was used to activate MAPKinase 2/Erk2, unactive. Subsequent phosphorylation of MBP (13-104) was performed.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-703) and in bulk (cat#14-703-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
14-703M 04053252925399

Documentation

Produits & Applications associés

Alternative Packsize

Référence Description
14-703 Cot1 Protein, active, 10 µg

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Catégories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases