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233153 Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Dithiothreitol, DTT

233153
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Aperçu

Replacement Information

Tableau de caractéristiques principal

CAS #Empirical Formula
3483-12-3C₄H₁₀O₂S₂

Products

RéférenceConditionnement Qté
233153-10GM Ampoule plast. 10 gm
233153-1GM Ampoule plast. 1 gm
233153-5GM Ampoule plast. 5 gm
Description
OverviewA protective agent for SH groups.
Absorbance: (100 mM, 280 nm):≤0.1. Oxidized dithiothreitol:≤0.5%.
Catalogue Number233153
Brand Family Calbiochem®
SynonymsDithiothreitol, DTT
References
ReferencesChau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
Shaked, Z., et al. 1980. Biochemistry 19, 4156.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.
Product Information
CAS number3483-12-3
FormWhite solid
Hill FormulaC₄H₁₀O₂S₂
Chemical formulaC₄H₁₀O₂S₂
Quality LevelMQ100
Applications
Biological Information
Purity≥99% by titration
Physicochemical Information
ContaminantsHeavy metals: <1 ppm
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSEK1610000
Safety Information
R PhraseR: 22-36/38

Harmful if swallowed.
Irritating to eyes and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
233153-10GM 04055977217650
233153-1GM 04055977217643
233153-5GM 07790788054267

Documentation

Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem Certificats d'analyse

TitreNuméro de lot
233153

Références bibliographiques

Aperçu de la référence bibliographique
Chau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
Shaked, Z., et al. 1980. Biochemistry 19, 4156.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2018 JSW
SynonymsDithiothreitol, DTT
DescriptionDTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes, protecting protein sulfhydryls and restoring enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
CAS number3483-12-3
RTECSEK1610000
Chemical formulaC₄H₁₀O₂S₂
Purity≥99% by titration
ContaminantsHeavy metals: <1 ppm
SolubilityEthanol or H₂O (200 mM)
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
Merck USA index14, 3376
ReferencesChau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
Shaked, Z., et al. 1980. Biochemistry 19, 4156.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.