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233155 Cleland’s Reagent - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: DTT, Dithiothreitol

233155
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Aperçu

Replacement Information

Tableau de caractéristiques principal

CAS #Empirical Formula
3483-12-3C₄H₁₀O₂S₂

Products

RéférenceConditionnement Qté
233155-100GM Flacon en verre 100 gm
233155-10GM Ampoule plast. 10 gm
233155-1KG Flacon en verre 1 kg
233155-25GM Flacon en verre 25 gm
233155-500GM Flacon en verre 500 gm
233155-5GM Ampoule plast. 5 gm
Description
OverviewA protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.
Oxidized dithiothreitol: ≤0.5%.
Catalogue Number233155
Brand Family Calbiochem®
SynonymsDTT, Dithiothreitol
References
ReferencesGailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.
Product Information
CAS number3483-12-3
FormWhite to off-white powder
Hill FormulaC₄H₁₀O₂S₂
Chemical formulaC₄H₁₀O₂S₂
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by titration
Physicochemical Information
ContaminantsHeavy metals: ≤1 ppm
Heavy metals<1 ppm
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSEK1610000
Safety Information
R PhraseR: 22-36/38

Harmful if swallowed.
Irritating to eyes and skin.
S PhraseS: 36-26

Wear suitable protective clothing.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
233155-100GM 07790788054274
233155-10GM 04055977217698
233155-1KG 04055977217681
233155-25GM 04055977217711
233155-500GM 04055977217735
233155-5GM 04055977217728

Documentation

Cleland’s Reagent - CAS 3483-12-3 - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Cleland’s Reagent - CAS 3483-12-3 - Calbiochem Certificats d'analyse

TitreNuméro de lot
233155

Références bibliographiques

Aperçu de la référence bibliographique
Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.

Brochure

Titre
Bulk Product Guide
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-April-2008 RFH
SynonymsDTT, Dithiothreitol
DescriptionDTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.
FormWhite to off-white powder
Intert gas (Yes/No) Packaged under inert gas
CAS number3483-12-3
RTECSEK1610000
Chemical formulaC₄H₁₀O₂S₂
Purity≥97% by titration
ContaminantsHeavy metals: ≤1 ppm
SolubilityEthanol or H₂O (30 mg/ml)
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
Merck USA index14, 3376
ReferencesGailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.