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218701 Casein Kinase II, Human, Recombinant, E. coli

Casein Kinase II, Human, Recombinant, E. coli Recombinant, is expressed in E. coli. A cyclic nucleotide-independent Ser/Thr protein kinase composed of catalytic α-subunits and regulatory β-subunits.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: rhCKII

218701
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
218701-10KU Ampoule plast. 10 ku
Description
OverviewRecombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits (M.W. 44 kDa) and regulatory β-subunits (M.W. 26 kDa) in an α2β2-tetramer. This material corresponds to one of several forms of native CKII. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies.
Note: 1 KU = 1000 units.
Catalogue Number218701
Brand Family Calbiochem®
SynonymsrhCKII
References
ReferencesYamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.
FormLiquid
FormulationIn 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON® X-100 Detergent, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
218701-10KU 04055977202519

Documentation

Casein Kinase II, Human, Recombinant, E. coli FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Casein Kinase II, Human, Recombinant, E. coli Certificats d'analyse

TitreNuméro de lot
218701

Références bibliographiques

Aperçu de la référence bibliographique
Yamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-July-2024 JSW
SynonymsrhCKII
DescriptionRecombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits (M.W. 44 kDa) and regulatory β-subunits (M.W. 26 kDa) in an α2β2- tetramer. This material is one of several forms of native CK-II. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies. The following substrates have been characterized by CK-II both in vitro and in vivo: nucleolin, RNA polymerase, p53, and SV 40 large T antigen.
FormLiquid
FormulationIn 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON® X-100 Detergent, pH 7.5.
Recommended reaction conditions20 mM Tris-HCl, pH 7.5, 50 mM KCl, 10 mM MgCl2, and 200 μM ATP (200 μCi/μmol). GTP can be used as a phosphoryl donor in place of ATP. Incubate at 30°C. Note: optimal incubation times and enzyme concentrations must be determined empirically for each substrate.
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesYamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.