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70794 BugBuster® GST•Bind™ Purification Kit

Affinity purification of GST fusion proteins

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
70794
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
70794-3 Flacon en verre 1 kit
Description
Overview

The BugBuster® GST•Bind™ Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.
Catalogue Number70794
Brand Family Novagen®
References
Product Information
•2 x 100 mlBugBuster Protein Extraction Reagent
•10,000 UBenzonase Nuclease, purity > 90%
•10 mlGST•Bind Resin
•pkg/4Chromatography Columns
•2 x 100 ml10X GST Bind/Wash Buffer
•40 ml10X Glutathione Reconstitution Buffer
•1 gGlutathione, Reduced
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 26

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
70794-3 04055977273236

Documentation

BugBuster® GST•Bind™ Purification Kit FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

BugBuster® GST•Bind™ Purification Kit Certificats d'analyse

TitreNuméro de lot
70794

Citations

Titre
  • Xiaobing Chen, et al. (2008) Organization of the core structure of the postsynaptic density. Procedings of the National Academy of Science 105, 4453-4458.
  • Bryan T. Carroll, et al. (2006) Sulfated signal from ASJ sensory neurons modulates stomatin-dependent coordination in Caenorhabditis elegans. Journal of Biological Chemistry 281, 35989-35996.
  • Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology 46, 1073-1082.
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    Protocoles Utilisateur

    Titre
    TB235 GST•Bind™ Kits
    TB245 BugBuster® Protein Extraction Reagent