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197217 Bcl-xL BH4₄₋₂₃, Human, Cell-Permeable - Calbiochem

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: HIV-TAT₄₈₋₅₇-β-Ala-Bcl-xL BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH

197217
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Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
197217-1MG Ampoule plast. 1 mg
Description
OverviewA cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Leads to the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-xL (amino acids 4 - 23) that has been shown to be essential for inhibiting VDAC activity in liposomes and in isolated mitochondria. The BH4 domain is linked to a carrier peptide, a 10-amino acid HIV-TAT48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized to the mitochondria.
Catalogue Number197217
Brand Family Calbiochem®
SynonymsHIV-TAT₄₈₋₅₇-β-Ala-Bcl-xL BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
References
ReferencesChen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem. 276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 3100.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₆₅H₂₇₉N₅₉O₄₆
Chemical formulaC₁₆₅H₂₇₉N₅₉O₄₆
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetVoltage-dependent anion channel (VDAC)
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceAc-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
197217-1MG 04055977206623

Documentation

Bcl-xL BH4₄₋₂₃, Human, Cell-Permeable - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Bcl-xL BH4₄₋₂₃, Human, Cell-Permeable - Calbiochem Certificats d'analyse

TitreNuméro de lot
197217

Références bibliographiques

Aperçu de la référence bibliographique
Chen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem. 276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 3100.

Brochure

Titre
Caspases and other Apoptosis Related Tools Brochure
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-July-2007
SynonymsHIV-TAT₄₈₋₅₇-β-Ala-Bcl-xL BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
DescriptionA highly cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Ultimately results in the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-xL (amino acids 4-23) that has been shown to be essential for inhibiting VDAC activity in liposomes and isolated mitochondria. The BH4 domain is linked to the carrier peptide, the 10-amino acid HIV-TAT48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized in the mitochondria.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₆₅H₂₇₉N₅₉O₄₆
Peptide SequenceAc-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml) or 20% Acetic Acid (5 mg/ml)
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesChen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem. 276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 3100.