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04-079-S Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size, rabbit monoclonal

Detect trimethyl-Histone H4 (Lys20) using this Anti-trimethyl-Histone H4 (Lys20) Antibody, rabbit monoclonal demontrated performance in WB, ChIP, & DB.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones, Chromatin Biology Gene Symbol: HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E UniProt Number: P62805
04-079-S
10 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, VrtWB, DB, ChIPRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-079-S
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size, rabbit monoclonal
Alternate Names
  • H4K20me3
  • Histone H4 (tri methyl K20)
Background InformationHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.
References
Product Information
FormatCulture Supernatant
PresentationRabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect trimethyl-Histone H4 (Lys20) using this Anti-trimethyl-Histone H4 (Lys20) Antibody, rabbit monoclonal demontrated performance in WB, ChIP, & DB.
Key Applications
  • Western Blotting
  • Dot Blot
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 10 µL of either a negative control supernatant, or Anti-Trimethyl-Histone H4 (Lys20) antibody and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of trimethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using ChIP Primers B-Globin. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-079-S, Anti-trimethyl Histone H4 (Lys20) (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Biological Information
ImmunogenPeptide corresponding to human Histone H4 containing the sequence [HRmethKVL] on which lysine 20 is trimethylated.
HostRabbit
SpecificityRecognizes Histone H4 when trimethylated on Lys20.
IsotypeIgG
Species Reactivity
  • Human
  • Vertebrates
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state..
Gene Symbol
  • HIST1H4A
  • H4/J
  • HIST1H4F
  • HIST1H4L
  • H4FN
  • H4FA
  • H4FH
  • HIST1H4H
  • H4F2
  • H4FM
  • H4/A
  • H4FK
  • H4FG
  • HIST2H4
  • H4FB
  • H4/K
  • HIST1H4I
  • H4/N
  • HIST1H4B
  • H4FE
  • H4/M
  • H4/a
  • HIST1H4E
  • HIST4H4
  • HIST2H4A
  • H4FC
  • H4FI
  • H4/H
  • HIST1H4C
  • H4/G
  • HIST1H4K
  • H4FJ
  • H4FD
  • H4/I
  • H4/B
  • H4/D
  • H4/C
  • HIST1H4J
  • HIST1H4D
  • H4/E
Modifications
  • Methylation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Molecular Weight11kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C from date of shipment
Packaging Information
Material Size10 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
04-079-S 04053252929854

Documentation

Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size, rabbit monoclonal FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size, rabbit monoclonal Certificats d'analyse

TitreNuméro de lot
Anti-trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Trial Size - 3317813 3317813
Anti-trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Trial Size - Q2290323 Q2290323
Anti-trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Trial Size -2767212 2767212
Anti-trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Trial Size Monoclonal Antibody 2876717