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MABE941 Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody

This Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody is validated for use in Western Blotting and Dot Blot and Immunocytochemistry and ELISA and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of phospho Histone H3.1 (Ser28).

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ
UniProt Number: P68431
MABE941
100 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, DB, ICC, ELISA, ChIP, ChIP-seqRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE941
ReplacesMABE13
DescriptionAnti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody
Alternate Names
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/I
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody is validated for use in Western Blotting and Dot Blot and Immunocytochemistry and ELISA and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of phospho Histone H3.1 (Ser28).
Key Applications
  • Western Blotting
  • Dot Blot
  • Immunocytochemistry
  • ELISA
  • Chromatin Immunoprecipitation (ChIP)
  • ChIP-seq
Application NotesChromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to be suitable for ChIP. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.

ChIP-seq: representative lot of this antibody was reported to be suitable for ChIP-seq. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.

Dot Blot Specificity Analysis: A representative lot of this antibody at a concentration of 0.5ug/mL was evaluated for cross reactivity with a panel of modified and unmodified peptides.

Immunocytochemistry: A representative lot of this antibody was reported to detect Histone H3.1 phosphorylated at serine 28 in Mitotic DM4 cells. See Yoshimi et al. (2013)
ELISA: A representative lot of this antibody was used in ELISA against a panel of phophorylated and unmodified peptides to demonstrate specificity. See Yoshimi et al. (2013).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human phospho Histone H3.1 (Ser28).
Clone5D10D4
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~ 17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in a nocodozole treated HeLa cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected phospho Histone H3.1 (Ser28) in a nocodozole treated HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABE941 04055977112139

Documentation

Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody Certificats d'analyse

TitreNuméro de lot
Anti-phospho Histone H3.1 (Ser28), -2776225 2776225
Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 - 3071357 3071357
Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 - 3460497 3460497
Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 - 3860855 3860855
Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 - 4181439 4181439
Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Monoclonal Antibody Q2475128

Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
A panel of specific monoclonal antibodies directed against various phosphorylated histones H3.
Yoshimi, T; Ohkawa, Y; Azuma, M; Tachibana, T
Monoclonal antibodies in immunodiagnosis and immunotherapy  32  119-24  2013

Afficher le résumé 		23607347 23607347