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ABS1008 Anti-phospho Glucocorticoid Receptor (Ser134) Antibody

Anti-phospho Glucocorticoid Receptor (Ser134) Antibody is an antibody against phospho Glucocorticoid Receptor for use in Western Blotting.

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Research Category: Signaling Research Sub-Category: Hormones & Receptors Gene Symbol: NR3C1, GCCR, GCR, GCRST, GRL UniProt Number: P04150
ABS1008
100 μg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1008
DescriptionAnti-phospho Glucocorticoid Receptor (Ser134) Antibody
Alternate Names
  • Glucocorticoid receptor, Ser134-phosphorylated
  • GR, Ser134-phosphorylated
  • Nuclear receptor subfamily 3 group C member 1, Ser134-phosphorylated
Background InformationGlucocorticoid receptor (UniProt P04150; also known as GR, Nuclear receptor subfamily 3 group C member 1) is encoded by the NR3C1 (also known as GCCR, GCR, GCRST, GRL) gene (Gene ID 2908) in human. Glucocorticoid receptor is widely expressed in almost all cell types and regulates genes that control development, metabolism, and immune response upon binding glucocorticoids. Hormone-bound GR translocates from the cytoplasm to the nucleus where it regulates gene transcriptions by binding to glucocorticoid response elements (GREs) or by interacting with various transcription factors. Alternatively, hormone-bound GR can represses gene transcriptions by blocking the nuclear translocation of transcription factors. BDNF-stimulated TrkB activation is shown to induce GR phosphorylation at Ser155 and Ser287 in primary rat cortical neurons (equivalent to human GR Ser134 and Ser267), resulting in enhanced GR transcription activity. GR is composed of an N-terminal modulating domain (a.a. 1-420), a DNA-binding domain (a.a. 421-486), and a C-terminal ligand-binding domain (a.a. 528-777).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in Tris-buffered saline (pH 8.0) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho Glucocorticoid Receptor (Ser134) Antibody is an antibody against phospho Glucocorticoid Receptor for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of endogenous GR in primary rat cortical neurons (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).
Western Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of exogenously expressed wild-type, but not S155A mutant, rat GR in transfected HEK293TrkB cells (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).
Biological Information
ImmunogenLinear peptide corresponding to a human glucocorticoid receptor sequence containing phosphorylated Ser134.
EpitopepSer134.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThe target phosphorylation site recognized by this polyclonal antibody is present in all 9 human GR spliced isoforms (Ser108 of isoforms Alpha-B and Beta-B; Ser134 of all the other isoforms) and in both rat GR spliced isoforms (Ser155 of isoform A and Ser128 of isoform B) reported by UniProt (P04150 and P06536, respectively).
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat. Predicted to react with Canine, Horse, Feline, Sheep based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NR3C1
  • GCCR
  • GCR
  • GCRST
  • GRL
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~85 kDa observed. 87.56 kDa (Rat isoforms A), 84.83 kDa (Rat isoforms B), 85.66 kDa (Human isoform Alpha; Alpha-A), 81.51 kDa (Human isoform Beta; Beta-A), 85.82 kDa (Human isoform Alpha-2; Gamma), 81.67 kDa (Human isoform Beta-2), 64.75 kDa (Human isoform GR-A alpha), 60.60 kDa (Human isoform GR-A beta), 82.85 kDa (Human isoform Alpha-B), 78.70 kDa (Human isoform Beta-B), 82.90 kDa (Human isoform 10; hGRDelta313-338) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in cell lysate from HEK293 expressing FLAG-tagged human glucocorticoid receptor.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Ser134 phosphorylation of FLAG-tagged human glucocorticoid receptor in 10 ug lysate from BDNF-treated HEK293 transfectants.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABS1008 04055977334234