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ABE2897-100UG Anti-phospho GATA6 (Ser37)

Anti-phospho GATA6 (Ser37), Cat. No. ABE2897, is a highly specific rabbit polyclonal antibody that targets Transcription factor GATA-6 (GATA-binding factor 6) and has been tested for use in Dot Blot, Immunoprecipitation, and Western Blotting.

Anti-phospho GATA6 (Ser37) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Gene Symbol: GATA6 UniProt Number: Q92908
ABE2897-100UG
100 μg  
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Replacement Information

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HDB, IP, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE2897-100UG
DescriptionAnti-phospho GATA6 (Ser37)
Alternate Names
  • GATA-binding factor 6
Background InformationTranscription factor GATA-6 (UniProt: Q92908; also known as GATA-binding factor 6) is encoded by the GATA6 gene (Gene ID: 2627) in human. GATA6 is a zinc-finger transcription factor that belongs to the six-member family of transcription factors that bind to the consensus sequence (A/T)GATA(A/G). GATA1, GATA2, and GATA3 are mainly expressed in hematopoietic cell lineages, while GATA4, GATA5, and GATA6 are predominantly expressed in the heart, gonads, and endodermal-derived tissues. It has two zinc-finger repeats localized to amino acids 390-414 and 444-468, which mediate interaction with LIM and cysteine-rich domains protein 1 (LMCD1). GATA-6 is shown to be important in the endodermal differentiation of organ tissues and has been indicated in proper lung development by controlling the late differentiation stages of alveolar epithelium and aquaporin-5 promoter activation. It has also been linked to the production of LIF that encourages proliferation of endodermal embryonic stem cells and blocks early epiblast differentiation. GATA6 is also required for definitive endoderm specification in human embryonic stem cells and induced pluripotent stem cells. Phosphorylation of GATA6 by GSK-3 on serine 37 is reported to promote its degradation, thereby preventing GATA6 from repressing transcripts that are induced by TNF and attenuated by insulin. Two isoforms of GATA-6 have been reported that are produced by alternative initiation. (Ref.: Chitforoushzadeh, Z., et al. (2016). ScienceSignaling 9 (431), ra59; Tiyaboonchai, A., et al. (2017). Stem Cell Reports 8 (3); 589-604).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-phospho GATA6 (Ser37), Cat. No. ABE2897, is a highly specific rabbit polyclonal antibody that targets Transcription factor GATA-6 (GATA-binding factor 6) and has been tested for use in Dot Blot, Immunoprecipitation, and Western Blotting.
Key Applications
  • Dot Blot
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected phospho GATA6 (Ser37) in 293T cells transfected with 3XFLAG-tagged GATA6L.

Western Blotting Analysis: A representative lot detected phospho GATA6 (Ser37) in Western Blotting applications (Chitforoushzadeh, Z., et. al. (2016). Sci Signal. 9(431):ra59).

Immunoprecipitation Analysis: 4 µg/mL from a representative lot immunoprecipitated phospho GATA6 (Ser37) in 293T cells were transfected with 3xFLAG-GATA6 (Courtesy of Dr. Kevin A. Janes at University of Virginia).

Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho GATA6 (Ser37) in Immunoprecipitation applications (Chitforoushzadeh, Z., et. al. (2016). Sci Signal. 9(431):ra59).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 37 of human Transcription factor GATA-6.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects GATA6 phosphorylated on Serine 37.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rat, Mouse, Porcine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GATA6
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~75 kDa observed; 60.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceDot Blot Analysis: A 1:500 dilution from a representative lot detected phospho GATA6 (Ser37) in Phosphorylated GATA6 peptide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABE2897-100UG 04054839516702