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OP03L Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
OP03L
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, Mk, M, Rb, RMMonoclonal Antibody

Products

RéférenceConditionnement Qté
OP03L-100UG Ampoule plast. 100 μg
Description
OverviewRecognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated p53.
Catalogue NumberOP03L
Brand Family Calbiochem®
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Kastan, M.B., et al. 1992. Cell 71, 587.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
Harlow, E., et al. 1981. J. Virol. 39 861.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Negative controlNormal skin tissue or SK-OV-3 cells
Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.
Key Applications Flow Cytometry
Frozen Sections
Gel Shift
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesFrozen Sections (10 µg/ml, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 µg per sample, see application references)
Application CommentsOriginally called clone L21. Does not recognize phosphorylated or acetylated p53. For a gel shift assay, resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then perform immunoblotting using Cat. No. PC35 and chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpartially purified mouse p53
ImmunogenMouse
Epitopewithin amino acids 376-378 of human p53
ClonePAb421
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Monkey
  • Mouse
  • Rabbit
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
OP03L-100UG 04055977209594

Documentation

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Certificats d'analyse

TitreNuméro de lot
OP03L

Références bibliographiques

Aperçu de la référence bibliographique
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Kastan, M.B., et al. 1992. Cell 71, 587.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
Harlow, E., et al. 1981. J. Virol. 39 861.

Brochure

Titre
Interactive Pathway Calendar
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-July-2008 JSW
ApplicationFrozen Sections (10 µg/ml, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 µg per sample, see application references)
DescriptionPurified mouse monoclonal antibody, originally called clone L21, generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
HostMouse
Immunogen speciesMouse
Immunogenpartially purified mouse p53
Epitopewithin amino acids 376-378 of human p53
ClonePAb421
IsotypeIgG2a
Specieshuman, monkey, mouse, rabbit, rat
Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Negative controlNormal skin tissue or SK-OV-3 cells
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
PreservativeNone
CommentsOriginally called clone L21. Does not recognize phosphorylated or acetylated p53. For a gel shift assay, resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then perform immunoblotting using Cat. No. PC35 and chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.
Toxicity Standard Handling
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Kastan, M.B., et al. 1992. Cell 71, 587.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
Harlow, E., et al. 1981. J. Virol. 39 861.
Application referencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.