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MABN2443-25UL Anti-nonphospho-GSK3 (Ser9) Antibody, beta specific Antibody, clone 12B2

Anti-nonphospho-GSK3 (Ser9), beta specific, clone 12B2, Cat. No. MABN2443, is a highly specific mouse monoclonal antibody that targets Glycogen synthase kinase-3 beta and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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Research Category: Neuroscience Gene Symbol: GSK3B UniProt Number: P49841
MABN2443-25UL
25 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MICC, IH(P), IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2443-25UL
DescriptionAnti-nonphospho-GSK3 (Ser9) Antibody, beta specific Antibody, clone 12B2
Alternate Names
  • Glycogen synthase kinase-3 beta
  • EC: 2.7.11.26
  • GSK-3 beta
  • Serine/threonine-protein kinase GSK3B
Background InformationGlycogen synthase kinase-3 beta (UniProt: P49841; also known as EC: 2.7.11.26, GSK-3 beta, Serine/threonine-protein kinase GSK3B) is encoded by the GSK3B gene (Gene ID: 2932) in human. GSK-3 is a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways during embryogenesis and in metabolic control. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3 alpha and a 47 kDa GSK-3 beta. The GSK-3 alpha contains a glycine-rich extension at its N-terminal. GSK-3 is constitutively active in cells and is regulated through inhibition of its activity. GSK-3 shows a preference for target proteins that are pre-phosphorylated at a `priming residue located C-terminal to the site of GSK-3 phosphorylation. Priming phosphorylation, although not absolutely required, enhances the efficiency of phosphorylation of most GSK-3 substrates. Phosphorylation of a threonine residue in the activation loop (T-loop) is considered to be essential for its activity. The T-loop of GSK-3 beta is phosphorylated at Tyr216, which play a role in forcing open the substrate-binding site of the enzyme. A primary mechanism of GSK-3 beta activity regulation is phosphorylation of N-terminal serine residues (Serine 9). Phosphorylation at this site is inhibitory to GSK-3 beta kinase activity because the phosphorylated N-terminus acts as a competitive inhibitor for primed substrates. GSK-3 beta is constitutively active in resting cells and treatment of cells with an agent, such as insulin, causes GSK-3 inactivation through a PI 3-kinase (PI 3-K)-dependent mechanism.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
Applications
ApplicationAnti-nonphospho-GSK3 (Ser9), beta specific, clone 12B2, Cat. No. MABN2443, is a highly specific mouse monoclonal antibody that targets Glycogen synthase kinase-3 beta and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected nonphospho-GSK3 (Ser9) in human and mouse brain tissue lysates.

ELISA Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in ELISA applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Western Blotting Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected nonphospho-GSK3 (Ser9) in human cerebral cortex tissue.

Immunocytochemistry Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Immunoprecipitation Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Immunohistochemistry Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).
Biological Information
ImmunogenKLH-conjugated three linear peptides from the N-terminal region of human GSK-3 beta.
Clone12B2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 12B2 detects GSK-3 beta in human, murine, and rat cells.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • GSK3B
Purification MethodProtein G purified
UniProt Number
Molecular Weight~45 kDa observed. 46.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected nonphospho-GSK3 (Ser9) in rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABN2443-25UL 04054839522178