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MABS277 Anti-monoglycylated Tubulin Antibody, clone TAP 952

MABS277
50 µL  
Purchase on Sigma-Aldrich

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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Dr, Su, Porcine, Sn, Sh, A Broad Range Of SpeciesWB, IF, DBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS277
DescriptionAnti-monoglycylated Tubulin Antibody, clone TAP 952
Background InformationSeveral post-translational modifications of tubulin have been identified in eukaryotic cells. Glycylation is a poly-modification which occurs as lateral branching of glycine chains of variable length identified in axonemal tubulin of Paramecium cilia. This modification has been detected on tubulin and/or cilia/flagella of many unicellular and pluricellular organisms. The differential distribution of distinct polyglycylated tubulin isoforms between cytoplasmic and axonemal compartments in Paramecium indicates that the polyglycylation level of tubulin is highly regulated at the cell level. In Paramecium, the TAP 952 antibody is reactive on cytoplasmic and axonemal tubulin. In metazoan cells and/or cell lines, it is specific for tubulin of motile cilia and primary cilia, and thus useful for motile cilia and primary cilia detection.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Tubulin using this mouse monoclonal antibody, Anti-monoglycylated Tubulin Antibody, clone TAP 952 validated for use in western blotting, Immunofluorescence & Dot Blot.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Dot Blot
Application NotesImmunofluorescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in mouse spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Dot Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in synthetic monoglycylated tubulin peptides (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Western Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in a panel of total protein extracts from select species (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Immunoflourescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in lemur, human, and sea urchin spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).
Biological Information
ImmunogenElectroeluted Paramecium axonemal tubulin
EpitopeAmino acids 427-449 of monoglycylated alpha-tubulin and 427-442 of monoglycylated beta-tubulin (Bré et al., 1998, Mol. Biol. Cell 9, 2655-2665)
CloneTAP 952
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the C-terminus of monoglycylated alpha and beta-tubulins.
Can be used as a marker for motile cilia.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Drosophila
  • Sea Urchin
  • Porcine
  • Snail
  • Sheep
  • A Broad Range Of Species
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular Weight~50 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting on Paramecium total cytoskeletal extract.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected monoglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABS277 04053252928000

Documentation

Anti-monoglycylated Tubulin Antibody, clone TAP 952 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-monoglycylated Tubulin Antibody, clone TAP 952 Certificats d'analyse

TitreNuméro de lot
Anti-monoglycylated Tubulin, clone -2805357 2805357
Anti-monoglycylated Tubulin, clone TAP 952 - 2535309 2535309
Anti-monoglycylated Tubulin, clone TAP 952 - 3307300 3307300
Anti-monoglycylated Tubulin, clone TAP 952 - 3589771 3589771
Anti-monoglycylated Tubulin, clone TAP 952 - 3764796 3764796
Anti-monoglycylated Tubulin, clone TAP 952 - 3890527 3890527
Anti-monoglycylated Tubulin, clone TAP 952 - 3989966 3989966
Anti-monoglycylated Tubulin, clone TAP 952 - 4043859 4043859
Anti-monoglycylated Tubulin, clone TAP 952 - Q2282925 Q2282925
Anti-monoglycylated Tubulin, clone TAP 952 -2626783 2626783

Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation.
Rogowski, Krzysztof, et al.
Cell, 137: 1076-87 (2009)  2009

Afficher le résumé
19524510 19524510
TTLL3 Is a tubulin glycine ligase that regulates the assembly of cilia.
Wloga, Dorota, et al.
Dev. Cell, 16: 867-76 (2009)  2009

Afficher le résumé
19531357 19531357
Polyglutamylation and polyglycylation of alpha- and beta-tubulins during in vitro ciliated cell differentiation of human respiratory epithelial cells.
Million, K, et al.
J. Cell. Sci., 112 ( Pt 23): 4357-66 (1999)  1998

Afficher le résumé
10564653 10564653
Tubulin polyglycylation: differential posttranslational modification of dynamic cytoplasmic and stable axonemal microtubules in paramecium.
Bré, M H, et al.
Mol. Biol. Cell, 9: 2655-65 (1998)  1998

Afficher le résumé
9725918 9725918
Axonemal tubulin polyglycylation probed with two monoclonal antibodies: widespread evolutionary distribution, appearance during spermatozoan maturation and possible function in motility.
Bré, M H, et al.
J. Cell. Sci., 109 ( Pt 4): 727-38 (1996)  1996

Afficher le résumé
8718664 8718664
Isolation and characterization of libraries of monoclonal antibodies directed against various forms of tubulin in Paramecium.
Callen, A M, et al.
Biol. Cell, 81: 95-119 (1994)  1993

Afficher le résumé
7531532 7531532
Polyglycylation of tubulin: a posttranslational modification in axonemal microtubules.
Redeker, V, et al.
Science, 266: 1688-91 (1994)  1993

Afficher le résumé
7992051 7992051