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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ABE286 Sigma-AldrichAnti-acetyl-p53 (Lys120) Antibody

Anti-acetyl-p53 (Lys120) Antibody is a highly specific rabbit polyclonal antibody, that targets p53 & has been tested in Dot Blot & IP.

Anti-acetyl-p53 (Lys120) Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: TP53, P53 UniProt Number: P04637

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Tableau de caractéristiques principal

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	DB, IP	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABE286
Description	Anti-acetyl-p53 (Lys120) Antibody
Alternate Names	Cellular tumor antigen p53 Antigen NY-CO-13 Phosphoprotein p53 Tumor suppressor p53
Background Information	p53 is a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers.

References

Product Information
Format	Affinity Purified
Control	Non-acetylated and acetylated p53 peptides
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-acetyl-p53 (Lys120) Antibody is a highly specific rabbit polyclonal antibody, that targets p53 & has been tested in Dot Blot & IP.
Key Applications	Dot Blot Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in camptothecin treated HCT116 cell lysate. Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in p53 transfected H1299 human lung cotransfected with or without hMOF (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College). Immunoprecipitation Analysis: 1.5 µg or 4.5 µg from a representative lot immunoprecipitated endogenous acetyl-p53 in HCT116 cell lysates, which were treated with a vehicle (DMSO) or camptothecin (CPT) (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College). Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in H1299 cell lysates, which were transfected with either wild type p53, the 9 K/R mutant (all lysine residues except Lys120 mutated to arginine), the 10 K/R mutant (all lysine residues mutate including Lys 120), or empty vector, with or without hMOF (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College).

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the AXIN1 interacting domain of human p53 acetylated at Lys120.
Epitope	AXIN1 interacting domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes the AXIN1 interacting domain of p53 acetylated at Lys120.
Species Reactivity	Human
Species Reactivity Note	Demonstrated to react with Human. Predicted to react with Sheep, Porcine, Rabbit, and Canine based on 100% sequence homology. Other homologies: Mouse, Rat, Bovine, Feline, Zebrafish, and Equine (88% sequence homology).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_000537
Gene Symbol	TP53 P53
Purification Method	Affinity Purfied
UniProt Number	P04637
Molecular Weight	~43 kDa calculated. Many isoforms are known to exist. This antibody is predicted to react with isoform 1 (44 kDa), isoform 2 (38 kDa), isoform 3 (39 kDa), isoform 4 (39 kDa), isoform 5 (34 kDa), and isoform 6 (34 kDa).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Dot Blot in non-acetylated and acetylated p53 peptides. Dot Blot Analysis: 1 µg/mL of this antibody detected acetyl-p53 in acetylated p53 peptides
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Référence	GTIN
ABE286	04053252861260

Documentation

Anti-acetyl-p53 (Lys120) Antibody FDS

Titre
Fiche de données de sécurité des matériaux (FDS)

Anti-acetyl-p53 (Lys120) Antibody Certificats d'analyse

Titre	Numéro de lot
Anti-acetyl-p53 (Lys120) - Q2150356	Q2150356
Anti-acetyl-p53 (Lys120) - 2520377	2520377
Anti-acetyl-p53 (Lys120) - 3311810	3311810
Anti-acetyl-p53 (Lys120) - 3589764	3589764
Anti-acetyl-p53 (Lys120) - 3836594	3836594
Anti-acetyl-p53 (Lys120) - 3991546	3991546
Anti-acetyl-p53 (Lys120) -2779744	2779744
Anti-acetyl-p53 (Lys120) -2792424	2792424
Anti-acetyl-p53 (Lys120) -2831333	2831333
Anti-acetyl-p53 (Lys120) -2845110	2845110

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Catégories

Life Science Research > Antibodies and Assays > Primary Antibodies

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