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06-1358 Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody

Use Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody (Rabbit Polyclonal Antibody) validated in WB & DB to detect acetyl (Lys34) phospho (Ser36) Histone H1 also known as Histone H1b.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H1E, H1F4 UniProt Number: P10412
06-1358
200 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, HWB, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-1358
DescriptionAnti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody
Alternate Names
  • Histone H1.4
  • Histone H1b
Background InformationH1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.
References
Product Information
FormatAffinity Purified
Control
  • Calf thymus histone extract
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody (Rabbit Polyclonal Antibody) validated in WB & DB to detect acetyl (Lys34) phospho (Ser36) Histone H1 also known as Histone H1b.
Key Applications
  • Western Blotting
  • Dot Blot
Application NotesDot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with acetyl (Lys34) phospho (Ser36) Histone H1.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Histone H1 acetylated at Lys34 and phosphorylated at Ser36.
EpitopeAcetylated Lys34 and phosphorylated Ser36
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Histone H1 acetylated at Lys34 and phosphorylated at Ser36.
Species Reactivity
  • Bovine
  • Human
Species Reactivity NoteDemonstrated to react with Bovine and Human. Predicted to react with Canine and Rhesus macaque based on 100% sequence homology. Other homologies: Mouse and Rat (90% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H1E
  • H1F4
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~34 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in calf thymus histone extract.

Western Blot Analysis: A 1:37 dilution of this antibody detected Histone H1 on 10 µg of calf thymus histone extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
06-1358 04053252648458