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06-942-AF555 Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 555 Conjugate

Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 555 Conjugate, Cat. No. 06-942-AF555, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.

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Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: P68431
06-942-AF555
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RICC, FCRbAlexaFluor®555Polyclonal Antibody
Description
Catalogue Number06-942-AF555
DescriptionAnti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 555 Conjugate
Alternate Names
  • H3K9ac
  • Histone H3, Lys9-acetylated
  • H3 histone family, member T, Lys9-acetylated
  • Lys9-acetylated histone 3
  • histone cluster 3, Lys9-acetylated
  • Lys9-acetylated H3
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatAlexaFluor®555
PresentationPurified rabbit polyclonal antibody in PBS with 1.5% BSA with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 555 Conjugate, Cat. No. 06-942-AF555, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.
Key Applications
  • Immunocytochemistry
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 2 µL from a representative lot detected cell population with histone H3 Lys9 acetylation (H3K9ac) among one million HEK293 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected nuclear histone H3 Lys9 acetylation (H3K9ac) in A431, HeLa, and NIH/3T3 cells.

The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.
Biological Information
ImmunogenKLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of histone H3 with acetylated Lys9.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityHistone H3 acetylated at position 9.
Species Reactivity
  • Mouse
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Modifications
  • Acetylation
Purification MethodProtein A purified.
UniProt Number
Molecular Weight~17 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HUVECs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys9 acetylation (H3K9ac) in HUVECs.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
06-942-AF555 04054839056253