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MABD168 Anti-WNT1 Antibody, clone 10C8

Detect Wnt-1 using this mouse monoclonal antibody, Anti-WNT1 Antibody, clone 10C8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.

Anti-WNT1 Antibody, clone 10C8 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: INT1, WNT1 UniProt Number: P04628
MABD168
100 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IF, IHC, FCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABD168
DescriptionAnti-WNT1 Antibody, clone 10C8
Alternate Names
  • Proto-oncogene Wnt-1
  • Proto-oncogene Int-1 homolog
Background InformationWnt-1, also known as Proto-oncogene Wnt-1 or Proto-oncogene Int-1 homolog, was first identified as a proto-oncogene activated by viral insertion in mouse mammary tumors. The Wnt-1 gene encodes a member of a large family of secreted proteins that are cysteine-rich, glycosylated, and poorly soluble. Wnt-1 serves as a ligand for the RYK coreceptor, resulting in Wnt signaling. The Wnt signalling pathway mediates the levels of cytosolic beta-catenin, a multi-functional protein that associates with membrane-bound E-cadherin as well as several DNA-binding proteins, such as members of the TCF/LEF family. Additionally, it serves a ligand for members of the frizzled family of seven transmembrane receptors and plays a role in development of the central nervous system.
References
Product Information
FormatAscites
HS Code3002 15 90
Control
  • NIH/3T3, 3T3L1, and HeLa cell lysates
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Wnt-1 using this mouse monoclonal antibody, Anti-WNT1 Antibody, clone 10C8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry
  • Flow Cytometry
Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in HeLa and 3T3-L1 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in human adrenal tissue.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected WNT1 in HeLa cells.

Optimal working dilutions must be determined by end user.

Biological Information
ImmunogenPurified recombinant fragment of human WNT1 expressed in E. Coli.
Clone10C8
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • INT1
  • WNT1
Purification MethodUnpurified
UniProt Number
Molecular Weight~41 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3, 3T3L1, and HeLa cell lysates.

Western Blot Analysis: A 1:500-2,000 dilution of this antibody detected WNT1 in NIH/3T3, 3T3L1, and HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABD168 04053252916496