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MABN361 Anti-VAPA Antibody, clone 7E10.1

This Anti-VAPA Antibody, clone 7E10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of VAPA.

Anti-VAPA Antibody, clone 7E10.1 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: VAPA, VAP33 UniProt Number: Q9P0L0
MABN361
100 μg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN361
DescriptionAnti-VAPA Antibody, clone 7E10.1
Alternate Names
  • Vesicle-associated membrane protein-associated protein A
  • VAMP-A
  • VAMP-associated protein A
  • VAP-A
  • 33 kDa VAMP-associated protein
  • VAP-33
  • VAPA
Background InformationVesicle-associated membrane protein-associated protein A (UniProt Q9P0L0; also known as 33 kDa VAMP-associated protein, hVAP-33, VAMP-A, VAMP-associated protein A, VAP-33, VAP-A) is encoded by the VAPA (also known as VAP33) gene (Gene ID 9218) in human. VAMP-associated proteins (VAPs) are type IV membrane proteins that are well conserved among species. There exist three human VAPs encoded by two genes, with VAPA encoding VAP-A and VAPB encoding VAP-B and VAP-C. VAPs generally localize at the endoplasmic reticulum (ER), although they are also reported to localize at other subcellular organelles in some species and cell types. VAP-A contains a major sperm protein (MSP) domain at the N-terminal, followed by a coiled-coil domain, and a transmembrane (TM) domain. VAPs were shown to have important roles in non-vesicular lipid transport, lipid metabolism, the regulation of ER structure, and the unfolded protein response through MSP domain-mediated interaction with FFAT motifs. Oxysterol-binding protein (OSBP) is a cytosolic receptor of cholesterol and oxysterols. OSBP is recruited to the ER by binding to the MSP domain of VAP-A, a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-VAPA Antibody, clone 7E10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of VAPA.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 0.2 µg/mL from a representative lot detected VAPA in 10 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected VAPA in human cerebellum and human testis tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminal half of human VAPA.
EpitopeC-terminal half
Clone7E10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityCross-reactivity to human VAPA spliced isoform 2 is highly expected, but not confirmed.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • VAPA
  • VAP33
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~30 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected VAPA in 10 µg of SH-SY5Y cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABN361 04055977276992

Documentation

Anti-VAPA Antibody, clone 7E10.1 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-VAPA Antibody, clone 7E10.1 Certificats d'analyse

TitreNuméro de lot
Anti-VAPA, clone 7E10.1 - 3523217 3523217
Anti-VAPA, clone 7E10.1 - 4012137 4012137
Anti-VAPA, clone 7E10.1 -Q2595829 Q2595829

Informations techniques

Titre
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.