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MABC945 Anti-TRIM3 Antibody, clone 13D12.1

Anti-TRIM3 Antibody, clone 13D12.1 is an antibody against TRIM3 Antibody for use in Western Blotting and Immunohistochemistry.

Anti-TRIM3 Antibody, clone 13D12.1 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: TRIM3, BERP, RNF22, RNF97 UniProt Number: O75382
MABC945
100 μg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC945
Description Anti-TRIM3 Antibody, clone 13D12.1
Alternate Names
  • Tripartite motif-containing protein 3
  • Brain-expressed RING finger protein
  • RING finger protein 22
  • RING finger protein 97
  • TRIM3
Background InformationTripartite motif-containing protein 3 (UniProt O75382; also known as Brain-expressed RING finger protein, RING finger protein 22, Ring finger protein 97, Tripartite motif protein TRIM3, Tripartite motif-containing 3) is encoded by the TRIM3 (also known as BERP, HAC1, RNF22, RNF97) gene (Gene ID 10612) in human. TRIM3 belongs to a family of more than 70 TRIM/RBCC proteins characterized by their shared tripartite motif structure, also known as the RBCC (RING finger, B-box, and coiled-coil) domain. The B-box and coiled coil regions are believed to participate in protein-protein interactions and formation of macromolecular complexes. TRIM proteins are involved in diverse cellular processes, including cell proliferation, differentiation, oncogenesis, and apoptosis. The presence of a RING domain suggests that TRIM proteins function as E3 ubiquitin ligases. TRIM3 is reported to catalyze the transfer of ubiquitin from UbcH5a to p21, a protein necessary for the proliferation of a subset of PDGF (platelet-derived growth factor)-transformed proneural glioma cells. Loss of TRIM3 heterozygosity is found in more than 10% of human GBMs (glioblastomas), while reducing TRIM3 expression facilitates tumor development and accelerates the progression of oligodendroglioma in a mouse model.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TRIM3 Antibody, clone 13D12.1 is an antibody against TRIM3 Antibody for use in Western Blotting and Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected TRIM3 in mouse brain and human skeletal muscle tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the internal fragment of human TRIM3.
EpitopeInternal
Clone13D12.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with all four spliced isoforms.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TRIM3
  • BERP
  • RNF22
  • RNF97
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~80 kDa observed. 80.8 kDa (Isoform 1), 79.6 kDa (Isoform 2), 72.1 kDa (Isoform 3), 67.7 kDa (Isoform 4) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TRIM3 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABC945 04055977294804