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ST1099 Anti-TORC2 (454-607) Rabbit pAb

This Anti-TORC2 (454-607) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of TORC2 (454-607).

Anti-TORC2 (454-607) Rabbit pAb : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2

ST1099
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

RéférenceConditionnement Qté
ST1099-50UL Flacon en verre 50 ul
Description
OverviewRecognizes the ~79-85 kDa TORC2 protein in primary hepatocytes, HepG2, NIH3T3, Min6, and FAO cells.
Catalogue NumberST1099
Brand Family Calbiochem®
SynonymsAnti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2
Application Data
Detection of rat TORC2 by immunoblotting. Sample: Whole cell lysate from primary rat hepatocytes. Primary antibody: Anti-TORC2 (454-607) Rabbit pAb (Cat. No. ST1099) (1:2000). Detection: chemiluminescence.
References
ReferencesScreaton, R.A., et al. 2004. Cell 119, 61.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlPrimary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:2000, see comments)
Immunoprecipitation (1:100, see comments)
Application CommentsThis antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ST1099-50UL 04055977224436

Documentation

Anti-TORC2 (454-607) Rabbit pAb FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-TORC2 (454-607) Rabbit pAb Certificats d'analyse

TitreNuméro de lot
ST1099

Références bibliographiques

Aperçu de la référence bibliographique
Screaton, R.A., et al. 2004. Cell 119, 61.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2007 RFH
SynonymsAnti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2
ApplicationImmunoblotting (1:2000, see comments)
Immunoprecipitation (1:100, see comments)
Application Data
Detection of rat TORC2 by immunoblotting. Sample: Whole cell lysate from primary rat hepatocytes. Primary antibody: Anti-TORC2 (454-607) Rabbit pAb (Cat. No. ST1099) (1:2000). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~79-85 kDa TORC2 protein.
BackgroundTORCs (Transducers Of Regulated cAMP Response Element-Binding (CREB) protein) are members of a conserved family of co-activators that enhance CRE-dependent transcription by a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB. Studies suggest that TORC phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP.
HostRabbit
Immunogen speciesHuman
Immunogena recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
IsotypeIgG
Specieshuman, mouse, rat
Positive controlPrimary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsThis antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesScreaton, R.A., et al. 2004. Cell 119, 61.