Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

574778 Anti-Synapsin I Rabbit pAb

This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of Synapsin I.

Anti-Synapsin I Rabbit pAb : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
574778
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
B, H, M, RRbPolyclonal Antibody

Products

RéférenceConditionnement Qté
574778-50UL Ampoule plast. 50 ul
Description
OverviewRecognizes the synapsin I protein in rat brain.
Catalogue Number574778
Brand Family Calbiochem®
References
ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlRat brain
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesELISA suitable recommend working dilution (1:2500-1:10,000)
Frozen Sections (1:5000)
Immunoblotting (1:200, chemiluminescence)
Immunocytochemistry suitable recommend working dilution (1:500-1:2500)
Immunoprecipitation suitable recommend working dilution (5 µl/200 µg total protein, see comments)
Application CommentsExhibits strong reactivity with the synapsin I doublet by immunoblotting. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. 5 µl will quantitatively immunoprecipitate all synapsin in 200 µg total protein from rat brain. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenpurified, bovine brain synapsin I
ImmunogenBovine
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
574778-50UL 04055977189742

Documentation

Anti-Synapsin I Rabbit pAb FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Synapsin I Rabbit pAb Certificats d'analyse

TitreNuméro de lot
574778

Références bibliographiques

Aperçu de la référence bibliographique
Stone, L.M., et al. 1994. J. Neurosci. 14, 301.
Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-July-2022 JSW
ApplicationELISA suitable recommend working dilution (1:2500-1:10,000)
Frozen Sections (1:5000)
Immunoblotting (1:200, chemiluminescence)
Immunocytochemistry suitable recommend working dilution (1:500-1:2500)
Immunoprecipitation suitable recommend working dilution (5 µl/200 µg total protein, see comments)
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the synapsin I protein.
BackgroundSynapsin I is a neuron specific protein that serves as an excellent marker for synaptic terminals. Can be used to estimate synaptic density and synaptogenesis.
HostRabbit
Immunogen speciesBovine
Immunogenpurified, bovine brain synapsin I
IsotypeIgG
Speciesbovine, human, mouse, rat
Positive controlRat brain
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsExhibits strong reactivity with the synapsin I doublet by immunoblotting. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. 5 µl will quantitatively immunoprecipitate all synapsin in 200 µg total protein from rat brain. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.