Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

574777 Anti-Synapsin I Rabbit pAb

This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.

Anti-Synapsin I Rabbit pAb : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
574777
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
B, H, M, RRbPolyclonal Antibody

Products

RéférenceConditionnement Qté
574777-10UG Ampoule plast. 10 μg
Description
OverviewRecognizes the synapsin I protein in rat brain.
Catalogue Number574777
Brand Family Calbiochem®
References
ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
Product Information
FormLyophilized
FormulationLyophilized from 5 mM ammonium bicarbonate.
Positive controlRat brain
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesFrozen Sections Andersson, S., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 3857.
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesELISA (1:2500-1:10,000)
Frozen Sections (see application references)
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate)
Application CommentsThis antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenpurified, brovine brain synapsin I
ImmunogenBovine
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
574777-10UG 04055977189735

Documentation

Anti-Synapsin I Rabbit pAb FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Synapsin I Rabbit pAb Certificats d'analyse

TitreNuméro de lot
574777

Références bibliographiques

Aperçu de la référence bibliographique
Stone, L.M., et al. 1994. J. Neurosci. 14, 301.
Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-July-2007 RFH
ApplicationELISA (1:2500-1:10,000)
Frozen Sections (see application references)
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate)
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.
BackgroundSynapsin I is a neuron-specific protein that serves as an excellent marker for synaptic terminals.
HostRabbit
Immunogen speciesBovine
Immunogenpurified, brovine brain synapsin I
IsotypeIgG
Speciesbovine, human, mouse, rat
Positive controlRat brain
FormLyophilized
FormulationLyophilized from 5 mM ammonium bicarbonate.
PreservativeNone
CommentsThis antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
Application referencesFrozen Sections Andersson, S., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 3857.