Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF2154-25UG Anti-Ricin B Chain Antibody, clone RBC11

Anti-Ricin B Chain, clone RBC11, Cat. No. MABF2154, is a mouse monoclonal antibody that detects Ricin B chain and has been tested for use in ELISA and Flow Cytometry.
May be used in antigen-capture sandwich ELISA when used combination with Cat. No. MABF2155; Anti-Ricin A chain Antibody, clone RAC18.

MABF2154-25UG
25 μg  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
Catalogue NumberMABF2154-25UG
DescriptionAnti-Ricin B Chain Antibody, clone RBC11
Alternate Names
  • RBC
Background InformationRicin is a dimeric toxin derived from Ricinus communis (Caster bean). It is composed of a sugar-binding subunit (B chain) that attached to cell surface receptors and facilitate the entry of toxin into the target cell and a second subunit (A chain), which has glycosidase activity that inactivates ribosomes by depurinating a single adenosine residue from an exposed loop of the 28S ribosomal RNA. The toxic effects of Ricin are attributed to the A chain. Ricin B chain is shown to be a lectin that binds to galactosides found at the cell surface in a non-cooperative manner. It has two Ricin-B type lectin domains (aa 7-134 and 137-261). The mode of galactose binding is reported to be nearly identical at the two sites. Clone RBC11 is shown to block binding of Ricin to the cell. (Ref.: Maddaloni, M., et al. (2004). J. Immunol. 172; 6221-6228; Rutenber, E., et al. (1987). Nature. 326(6113):624-626).
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Ricin B Chain, clone RBC11, Cat. No. MABF2154, is a mouse monoclonal antibody that detects Ricin B chain and has been tested for use in ELISA and Flow Cytometry.
May be used in antigen-capture sandwich ELISA when used combination with Cat. No. MABF2155; Anti-Ricin A chain Antibody, clone RAC18.
Key Applications
  • ELISA
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A representative lot detected Ricin B chain in Flow Cytometry applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).

ELISA Analysis: A representative lot detected Ricin B chain in ELISA applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8; Simon, S., et. al. (2015). Toxins (Basel) 7(12):4967-86).
Biological Information
ImmunogenPurified Ricin B chain.
CloneRBC11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone RBC 11 binds to Ricin B chain.
IsotypeIgG2bκ
Species Reactivity
  • Ricinus communis
Species Reactivity NoteRicinus communis.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight28.90 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with Ricin B chain.

ELISA Analysis: Various dilutions of this antibody detected Ricin B chain in an indirect ELISA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABF2154-25UG 04054839659935