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MAB4410-I-25UL Anti-Renilla Luciferase Antibody, clone 1D5.2

Anti-Renilla Luciferase, clone 1D5.2, Cat. No. MAB4410-I, is a mouse monoclonal antibody that detects Renilla-type luciferase (Renilla-luciferin 2-monooxygenase) and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

MAB4410-I-25UL
25 µL  
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Replacement Information
Description
Catalogue NumberMAB4410-I-25UL
DescriptionAnti-Renilla Luciferase Antibody, clone 1D5.2
Alternate Names
  • Coelenterazine h 2-monooxygenase
  • EC:1.13.12.5
  • Renilla-luciferin 2-monooxygenase
Background InformationCoelenterazine h 2-monooxygenase (UniProt: P27652; also known as EC:1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase) is a monomeric, bioluminescent protein derived from the sea pansy (Renilla reniformis). Due to its light emitting abilities, the luciferase from Renilla reniformis has been widely used in molecular biology as a reporter gene in cell culture experiments and small animal imaging. Renilla luciferase is shown to be present in membrane-bound intracellular structures within specialized light-emitting cells along with a closely interacting green fluorescent protein (RrGFP) and a calcium- activated luciferin-binding protein. Upon binding the substrate, it catalyzes an oxygenation reaction, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure that collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. The optimum pH for this reaction is 7.4. It is shown that under in vivo conditions the product undergoes the process of nonradiative energy transfer to an accessory protein, a GFP, which results in green bioluminescence (~ 509 nm). However, in vitro, in the absence of GFP, it emits blue light (~480 nm). (Ref.: Loening, AM., et al. (2007). J. Mol. Biol. 374(4); 1017-1028; Lorenz, WW., et al. (1991). Proc. Natl. Acad. Sci. USA 88(10); 4438-4442).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Renilla Luciferase, clone 1D5.2, Cat. No. MAB4410-I, is a mouse monoclonal antibody that detects Renilla-type luciferase (Renilla-luciferin 2-monooxygenase) and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.
Key Applications
  • ELISA
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Renilla Luciferase in Immunoprecipitation applications (Adelfinger, L., et. al. (2014). Biochem Pharmacol. 91(3):369-79).

Western Blotting Analysis: A representative lot detected Renilla Luciferase in Western Blotting applications (Simms, C.L., et. al. (2019). Cell Rep. 28(7):1679-1689; Lu, Q., et. al. (2018). Nat Commun. 9(1):3104).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged full-length recombinant Renilla reniformis Luciferase.
EpitopeUnknown
Clone1D5.2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5B11.2 is a mouse monoclonal antibody that detects Renilla-type luciferase (Renilla-luciferin 2-monooxygenase).
IsotypeIgG
Species Reactivity
  • All
Species Reactivity NoteRenilla reniformis.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Molecular Weight36.02 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with recombinant Renilla reniformis Luciferase.

ELISA Analysis (ELISA): Various dilution (starting at 5 µg/mL, two-fold serial dilutions; 8 points) of this antibody detected recombinant Renilla reniformis luciferase.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MAB4410-I-25UL 04065265944731