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MABS1234 Anti-Progesterone Receptor B Antibody, clone 250/H11

This Anti-Progesterone Receptor B Antibody, clone 250/H11 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor B.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PGR, NR3C3 UniProt Number: P06401
MABS1234
100 μg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1234
DescriptionAnti-Progesterone Receptor B Antibody, clone 250/H11
Alternate Names
  • Progesterone receptor
  • PR
  • Nuclear receptor subfamily 3 group C member 3
  • Progesterone Receptor B
Background InformationProgesterone receptor (UniProt P06401; also known as Nuclear receptor subfamily 3 group C member 3, PR) is encoded by the PGR (also known as NR3C3) gene (Gene ID 5241) in human. The progesterone receptor (PR) is a hormone activated transcription factor, where hormone binding triggers the dissociation of heat shock and chaperone proteins from PR, leading to PR dimerization and activation. The receptor dimer then binds progestin response elements in the regulatory regions of its target genes and a multi-component complex is assembled to enable transcription. In addition, a number of kinases are also reported to regulate PR activity via phosphorylation. PR can also regulate transcription without direct DNA binding by tethering to other transcription factors, such as specificity protein 1 (Sp1) or activator protein 1 (AP-1), and modulating their transcriptional activity on target genes. PR is expressed as five isoforms, including PR-B and PR-A that are produced as a result of alternate estrogen inducible promoters within the same PGR gene. PR-A lacks the first 164 amino acids of PR-B but the two isoforms have otherwise identical sequence. PR-B and PR-A can regulate distinct subsets of target genes and studies in knockout mice suggest that PR-B predominantly regulates mammary gland development and PR-A is critical for normal uterine function.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Progesterone Receptor B Antibody, clone 250/H11 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor B.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A representative lot stained formalin-fixed and paraffin-embedded MCF10A cells engineered to express progesterone receptor isoform B (PR-B), but not MCF10A cells engineered to express isoform A/PR-A (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Immunocytochemistry Analysis: A representative lot stained paraformaldehyde-fixed and Triton X-100-fixed MCF10A cells engineered to express progesterone receptor isoform B (PR-B), but not MCF10A cells engineered to express isoform A/PR-A by fluorescent immunocytochemistry (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Western Blotting Analysis: A representative lot detected endogenous progesterone receptor isoform B (PR-B) in human breast cancer T47D cell lysate, as well as exogenously expressed PR-B in transfected DCIS.COM human ductal carcinoma in situ (DCIS) breast cancer cells, but not in PR-A-transfected or untransfected DCIS.COM cells (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Biological Information
ImmunogenRecombinant protein corresponding to the N-terminal of human Progesterone Receptor B.
EpitopeN-terminal
Clone250/H11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 250/H11 reacts with isoform 1 (isoform B; PR-B), but not isoform 2 (isoform A; PR-A). This clone is also expected to react with isoform 5 (delta4), but not isoform 3 or 4 (PR-M).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PGR
  • NR3C3
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~110 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human breast cancer T47D cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected progesterone receptor isoforms B (PR-B), but not isoform A (PR-A) in human breast cancer T47D cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABS1234 04055977288391

Documentation

Anti-Progesterone Receptor B Antibody, clone 250/H11 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Progesterone Receptor B Antibody, clone 250/H11 Certificats d'analyse

TitreNuméro de lot
Anti-Progesterone Receptor B, -Q2606953 Q2606953
Anti-Progesterone Receptor B, clone 250/H11 - 3245597 3245597
Anti-Progesterone Receptor B, clone 250/H11 - 3682534 3682534

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