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MABS831 Anti-PI3 Kinase Antibody, p85 Antibody, N-SH3 Antibody, clone 1D5.1

Anti-PI3 Kinase, p85, N-SH3, clone 1D5.1, Cat. No. MABS831, is a mouse monoclonal antibody that detects human and murine p85 subunit of PI3 kinase. It has been tested for use in Western Blotting.

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Research Category: Signaling Gene Symbol: PIK3R1, GRB1 UniProt Number: P27986
MABS831
100 μg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS831
DescriptionAnti-PI3 Kinase Antibody, p85 Antibody, N-SH3 Antibody, clone 1D5.1
Alternate Names
  • Phosphatidylinositol 3-kinase regulatory subunit alpha
  • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
  • PI3-kinase regulatory subunit alpha
  • PtdIns-3-kinase regulatory subunit p85-alpha
  • PI3-kinase subunit p85-alpha
  • PI3K regulatory subunit alpha
  • PtdIns-3-kinase regulatory subunit alpha
Background InformationPhosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: P27986; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the PIK3R1 (also known as GRB1) gene (Gene ID: 5295) in human. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. PI3 kinase alpha plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in PIK3R1 gene are known to be associated with insulin resistance.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PI3 Kinase, p85, N-SH3, clone 1D5.1, Cat. No. MABS831, is a mouse monoclonal antibody that detects human and murine p85 subunit of PI3 kinase. It has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected PI3 Kinase, p85, N-SH3 in 10 µg of human cerebral cortex tissue lysate.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 77 amino acids from the N-terminal region of the p85 regulatory subunit of human PI 3-kinase alpha.
Clone1D5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1D5.1 detects p85 subunit of human and murine PI-3 kinase alpha. It targets an epitope within 77 amino acids from the N-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PIK3R1
  • GRB1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~85 kDa observed; 83.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 L1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 Kinase, p85, N-SH3 in 10 µg of NIH/3T3 L1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABS831 04054839224232