Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABN1486 Anti-PDE2A

Anti-PDE2A, Cat. No. ABN1486, is a highly specific rabbit polyclonal antibody that targets cGMP-dependent 3',5'-cyclic phosphodiesterase and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PDE2A : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Gene Symbol: PDE2A UniProt Number: O00408
ABN1486
100 μg  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1486
DescriptionAnti-PDE2A
Alternate Names
  • cGMP-dependent 3
  • 5'-cyclic
  • phosphodiesterase
  • PDE2A
  • Cyclic GMP-stimulated phosphodiesterase
  • cGSPDE
  • CGS-PDE
Background InformationcGMP-dependent 3',5'-cyclic phosphodiesterase (UniProt: O00408; also known as EC:3.1.4.17, PDE2A, Cyclic GMP-stimulated phosphodiesterase, CGS-PDE, cGSPDE) is encoded by the PDE2A gene ( Gene ID: 5138) in human. PDE2A is an evolutionarily conserved cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP that are key regulators of many important physiological processes. PDE2A is expressed in both the periphery and in the central nervous system, however its expression is highest in the brain, where it is mainly localized in the cortex, hippocampus, and striatum. Three splice variants of PDE2A have been described, the PDE2A1, PDE2A2 and PDE2A3. PDE2A1 is cytosolic whereas -A2 and -A3 are membrane bound. PDE2A isoform 2 is specifically targeted to the mitochondrial matrix, where it forms a local signaling system with soluble adenylate cyclase regulating the respiratory chain. PDE2A is a dimeric enzyme that binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. Each PDE2A monomer contains an N-terminal (200 residues) domain of unknown function, tandem GAF domains (GAF A and GAF B), and a C-terminal catalytic domain. GAF A functions as a dimerization locus. PDE2 A catalytic activity is allosterically stimulated by cGMP binding to its GAF B domain. Binding of cGMP induces conformational change in the protein structure leading to higher enzyme activity. Ref.: Acin-Perez, R., et al. (2011). J. Biol. Chem. 286 (35): 30423-30432; Martinez, SE., et al. (2002). Proc. Natl. Acad. Sci. USA 99(20); 13260-13265; Trabanco, A., et al. (2016). Expert Opin. Therap. Patents 26(8): 933-946).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PDE2A, Cat. No. ABN1486, is a highly specific rabbit polyclonal antibody that targets cGMP-dependent 3',5'-cyclic phosphodiesterase and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PDE2A in human cerebellum, human cerebral cortex, human skin, human lung, human kidney, and human heart tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of human PDE2A.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody specificaaly detects PDE2A in human.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PDE2A
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~106 kDa observed; 105.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PDE2A in 10 µg of human cerebral cortex tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABN1486 04054839193620