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MAB4092 Sigma-AldrichAnti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

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Références bibliographiques
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Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: SELPLG, CLA, PSGL-1, CD162, PSGL1 UniProt Number: Q14242

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Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, IHC, IH(P), IP, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB4092
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1
Alternate Names	PSGL-1 CD162
Background Information	Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.

References

Product Information
Format	Purified
Presentation	Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.
Quality Level	MQ100

Applications
Application	Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.
Key Applications	Flow Cytometry Immunohistochemistry Immunohistochemistry (Paraffin) Immunoprecipitation Western Blotting
Application Notes	Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000 FACS {Snapp, KR et al 1998}. Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein). Functional blocking of P-selectin {Snapp KR et al 1998}. Immunohistochemistry: Fresh frozen, acetone fixed sections Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200 Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).
Clone	KPL-1
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.
Isotype	IgG1κ
Species Reactivity	Human
Species Reactivity Note	Known not to react with Rat, Bovine, Pig, and Equine PSGL-1 {Snapp, KR et al 1998; Baisse, B et al 2007}.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_003006.3
Entrez Gene Summary	SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.
Gene Symbol	SELPLG CLA PSGL-1 CD162 PSGL1
UniProt Number	Q14242
UniProt Summary	FUNCTION: SwissProt: Q14242 # Binds to P-, E- and L-selectins. The calcium-dependent high affinity interaction with P-selectin mediates the tethering and rolling of neutrophils and T-lymphocytes on endothelial cells. SIZE: 412 amino acids; 43201 Da SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes. PTM: Displays complex, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Also N- glycosylated. & Sulfated in the N-terminal region; sulfation is necessary for P-selectin binding.

Physicochemical Information

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Safety Information according to GHS

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Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2-8°C in undiluted aliquots up to 12 months.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Référence	GTIN
MAB4092	04053252667763

Documentation

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 FDS

Titre
Fiche de données de sécurité des matériaux (FDS)

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 Certificats d'analyse

Titre	Numéro de lot
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY - 2383971	2383971
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY - 2359959	2359959
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3231554	3231554
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3680716	3680716
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3946452	3946452
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3959180	3959180
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 4135337	4135337
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2594838	2594838
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2614679	2614679
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2635046	2635046

Références bibliographiques

Aperçu de la référence bibliographique	Application	Nº PubMed
Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions. Long, M, et al. Annals of biomedical engineering, 29: 935-46 (2001) 2001 Afficher le résumé Selectin/ligand interactions initiate the multistep adhesion and signaling cascades in the recruitment of leukocytes from circulation to inflamed tissues and may also play a role in tumor metastasis. Kinetic properties of these interactions are essential determinants governing blood-borne cells' tethering to and rolling on the vessel wall. Extending our recently developed micropipette method, we have measured the kinetic rates of E-selectin/ligand interactions. Red cells coated with an E-selectin construct were allowed to bind HL-60 or Colo-205 cells bearing carbohydrate ligands. Specific adhesions were observed to occur at isolated points, the frequency of which followed a Poisson distribution. These point attachments were formed at the same rate with both the HL-60 and Colo-205 cells (0.14 +/- 0.04 and 0.13 +/- 0.03 microm2 s(-2) per unit density of E-selectin, respectively) but dissociated from the former at a rate twice as fast as did from the latter (0.92 +/- 0.23 and 0.44 +/- 0.10 s(-1), respectively). The reverse rates agree well with those measured by the flow chamber. The forward rates are orders of magnitude higher than those of Fcgamma receptors interacting with IgG measured under similar conditions, consistent with the rapid kinetics requirement for the function of E-selectin/ligand binding, which is to capture leukocytes on endothelial surfaces from flow.	Inhibits Activity/Function	11791676

Fiche technique

Titre
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY

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Catégories

Life Science Research > Antibodies and Assays > Primary Antibodies

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