Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS1967-25UL Anti-Osteocalcin (Glu17) Antibody, clone 4B6

Detects osteocalcin using this Anti-Osteocalcin (Glu17), clone 4B6, Cat. No. MABS1967, is a mouse monoclonal antibody that detects osteocalcin not carboxylated on Glu17 and has been tested for use in and ELISA and Dot Blot.

MABS1967-25UL
25 µL  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
Catalogue NumberMABS1967-25UL
DescriptionAnti-Osteocalcin (Glu17) Antibody, clone 4B6
Alternate Names
  • Bone Gla protein
  • BGP
  • Gamma-carboxyglutamic acid-containing protein
Background InformationOsteocalcin (UniProt: P02818; also known as Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein) is encoded by the BGLAP gene (Gene ID: 632) in human. Osteocalcin is a member of the osteocalcin/matrix Gla protein family and can constitute 1 to 2% of the total bone protein. It is synthesized by osteoblasts and is a major component of the noncollagenous bone matrix. Osteocalcin is synthesized with a signal peptide (aa 1-23), a propeptide (aa 24-51) that are cleaved to generate active secreted form of osteocalcin (aa 52-100). Osteocalcin displays a high affinity for calcium and binds strongly to apatite. Sixty to ninety percent of de novo synthesized osteocalcin is incorporated into the bone matrix where it binds to hydroxyapatite during matrix mineralization. The remainder of the osteocalcin is released into the circulation where it can be measured as a sensitive marker of bone formation. Osteocalcin found in serum is almost exclusively derived from the bone formation with little or no contribution from the resorption process. A major feature of osteocalcin is the presence of 3 vitamin K-dependent gamma-carboxyglutamic acid (Gla) residues, which are found in the first helical region and interact with the inter-calcium spacing in the HAP lattice. In humans, the percentage of the circulating osteocalcin that is not gamma-carboxylated is used as a biomarker of vitamin K status. Osteocalcin expression in osteoblasts is stimulated by insulin and adiponectin and osteocalcin enhances insulin sensitivity and differentiation in muscle. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget's disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency. (Ref.: Kanazawa I (2015). World J Diabetes. 6(18): 1345 1354; Gundberg, CM et al. (2012). Adv Nutr 3 (2): 149-157).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationDetects osteocalcin using this Anti-Osteocalcin (Glu17), clone 4B6, Cat. No. MABS1967, is a mouse monoclonal antibody that detects osteocalcin not carboxylated on Glu17 and has been tested for use in and ELISA and Dot Blot.
Key Applications
  • ELISA
  • Dot Blot
Application NotesDot Blot Analysis: A 1:250 dilution from a representative lot detected Osteocalcin (Glu17) at a wide range of concentrations (0.8 to 400 ng) (Courtesy of Mathieu Ferron, Ph.D., IRCM, Montreal, Canada).



ELISA Analysis: A representative lot detected Osteocalcin (Glu17) in an indirect ELISA (Courtesy of Mathieu Ferron, Ph.D., IRCM, Montreal, Canada).
Biological Information
ImmunogenA linear peptide corresponding to 17 amino acids from the central region of the mature human osteocalcin with decarboxylated GLU17 residue (with signal peptide and pro regions cleaved off).
Clone4B6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4B6 is a mouse monoclonal antibody that detects human osteocalcin only when not carboxylated on the first glutamic acid residue (GLU17). It does not react with carboxylated osteocalcin.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BGLAP
Purification MethodProtein G purified
UniProt Number
Molecular Weight10.96 kDa calculated (for complete molecule)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABS1967-25UL 04054839990694