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MABS1267 Anti-Nuclear Pore Complex Proteins Antibody, clone 414

Anti-Nuclear Pore Complex Proteins Antibody, clone 414 is an antibody against Nuclear Pore Complex Proteins for use in Immunocytochemistry, Immunoprecipitation, Western Blotting, Electron Microscopy.

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Research Category: Signaling Research Sub-Category: Chromatin Biology Gene Symbol: Nup62 UniProt Number: P17955
MABS1267
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Yeast, R, MICC, IP, WB, EMMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1267
DescriptionAnti-Nuclear Pore Complex Proteins Antibody, clone 414
Alternate Names
  • Nuclear pore glycoprotein p62
  • 62 kDa nucleoporin
  • Nucleoporin Nup62
  • Nuclear pore complex proteins
Background InformationNuclear pore glycoprotein p62 (UniProt P17955; also known as 62 kDa nucleoporin, Nucleoporin Nup62) is encoded by the Nup62 gene (Gene ID 65274) in rat species. The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. Soluble cargo-protein complexes navigate through the pore by binding to phenylalanine-glycine (FG)-repeat proteins attached to the channel walls. The Nup62 complex is located in the center of the NPC and contains the FG-repeat proteins Nup62, Nup54, and Nup58 associated with each other via conserved coiled-coil segments in a 1:1:1 stiochiometric ratio. The nuclear envelope breaks down during prometaphase. The disassembled nucleoporin subcomplexes act as critical regulators of various mitotic events to ensure proper chromosome segregation. Specifically, Nup62 is reported to localize to centrosomes/spindle poles, where it associates with the centrosomal proteins gamma-tubulin and hSAS-6. Nup62 is involved in the recruitment of critical proteins to the centrosome and its depletion results in mislocalization of several centrosomal components.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nuclear Pore Complex Proteins Antibody, clone 414 is an antibody against Nuclear Pore Complex Proteins for use in Immunocytochemistry, Immunoprecipitation, Western Blotting, Electron Microscopy.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
  • Electron Microscopy
Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot immunostained NIH/3T3 cell nuclear rim.
Immunocytochemistry Analysis: A representative lot immunostained yeast nuclear envelope with a punctate and patchy pattern (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067).
Immunocytochemistry Analysis: Representative lots detected a punctate staining pattern of Nup62 at the nuclear rim by fluorescent immunocytochemistry using 2% formaldehyde-fixed, methanol-permeabilized Buffalo rat liver (BRL) cells (Davis, L.I., and Blobel, G. (1987). Proc. Natl. Acad. Sci. U. S. A. 84(21):7552-755; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
Electron Microscopy: A representative lot immunostained yeast nuclear envelope using 3% paraformaldehyde/0.2% glutaraldehyde-fixed yeast nuclei LR White sections (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067).
Electron Microscopy: A representative lot immunostained the pore complexes in thin sections of isolated rat liver nuclei extracted with 2% Triton X-100 and fixed with 0.05% glutaraldehyde (Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
Immunoprecipitation Analysis: A representative lot immunoprecipited a ~100 kDa (p110) and a ~95 kDa (p95) protein species from yeast nuclear extract. An additional ~55 kDa protein was immunoprecipitated by clone 414 using yeast cytosolic fraction or whole cell lysate (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067).
Immunoprecipitation Analysis: A representative lot immunoprecipitated three major GlcNAcylated prtoein species of 62, 175, and 270 kDa from SDS-solubilized pore complex-lamina extract of Buffalo rat liver (BRL) cell nuclei preparation that had been labeled with UDP-[3H]Gal by galactosyltransferase (Davis, L.I., and Blobel, G. (1987). Proc. Natl. Acad. Sci. U. S. A. 84(21):7552-755).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GlcNAcylated 62 kDa protein (p62) from the SDS-solubilized pore complex-lamina extract, as well as a less glycosylated p61 cytoplasmic form from the postmitochondrial supernatant of Buffalo rat liver (BRL) cells (7552-755; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
Western Blotting Analysis: A representative lot detected a ~100 kDa (p110) and a ~95 kDa (p95) immunoreactive bands in yeast nuclear extract (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067).
Western Blotting Analysis: A representative lot detected a ~62 kDa (p62) and a ~200 kDa target bands associated with nuclear pore complex-lamina of rat liver nuclei preparation even following sequential nucleaases, Triton X-100, and 140 mM NaCl treatments (Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
Biological Information
ImmunogenTriton X-100 treated rat liver nuclei.
Clone414
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 414 immunostained rat liver cell nuclear rim and yeast cell nuclear envelope (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
IsotypeIgG1κ
Species Reactivity
  • Human
  • Yeast
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nup62
Purification MethodProtein G Purified
UniProt Number
Molecular Weight53.40 kDa (Rat Nup62) calculated. ~62/175/270 kDa (Rat liver nuclear extract) and ~95/100 kDa (Yeast nuclear extract) reported (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709). Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:200 dilution of this antibody immunostained HeLa cell nuclear rim.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABS1267 04055977349740

Documentation

Anti-Nuclear Pore Complex Proteins Antibody, clone 414 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Nuclear Pore Complex Proteins Antibody, clone 414 Certificats d'analyse

TitreNuméro de lot
Anti-Nuclear Pore Complex Proteins, clone 414 - 3514181 3514181
Anti-Nuclear Pore Complex Proteins, clone 414 - 3788632 3788632
Anti-Nuclear Pore Complex Proteins, clone 414 - 3953426 3953426
Anti-Nuclear Pore Complex Proteins, clone 414 - 3986180 3986180
Anti-Nuclear Pore Complex Proteins, clone 414 -Q2676868 Q2676868