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MAB5256A5 Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor® 555 Conjugate

Detect Neurofilament 200kDa using this Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate validated for use in ICC & IHC.

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Research Category: Neuroscience Research Sub-Category: Neurofilament & Neuron Metabolism Gene Symbol: NEFH, NFH UniProt Number: P12036
MAB5256A5
100 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, M, Po, HICC, IHCMAlexaFluor®555Monoclonal Antibody
Description
Catalogue NumberMAB5256A5
DescriptionAnti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor® 555 Conjugate
Alternate Names
  • Neurofilament heavy polypeptide
  • NF-H
  • 200 kDa neurofilament protein
  • Neurofilament triplet H protein
Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information and epitope maps, search "neurofilament" on our website.
Product Information
FormatAlexaFluor®555
Control
  • Rat E18 primary cortex cells
PresentationPurified mouse monoclonal IgG1 conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationDetect Neurofilament 200kDa using this Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate validated for use in ICC & IHC.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Neurofilament in adult mouse brain cryosections.
Biological Information
ImmunogenPurified neurofilament polypeptides (Debus et al., 1983).
CloneNE14
HostMouse
IsotypeIgG1
Species Reactivity
  • Rat
  • Mouse
  • Pig
  • Human
Species Reactivity NoteDemonstrated to react with Mouse, and Rat. Predicted to react with Porcine based on immunogen design. Predicted to react with Human based on 100% sequence homology. Unconjugated Anti-Neurofilament has been shown to work with multiple species including Human and Porcine.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NEFH
  • NFH
Purification MethodProtein A Purfied
UniProt Number
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Référence GTIN
MAB5256A5 04053252695100