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MABT416 Anti-Nephrin Antibody, clone 3B6.1

Anti-Nephrin Antibody, clone 3B6.1 is an antibody against Nephrin for use in Western Blotting.

Anti-Nephrin Antibody, clone 3B6.1 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Renal Physiology Gene Symbol: NPHS1, NPHN UniProt Number: O60500
MABT416
100 μg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT416
DescriptionAnti-Nephrin Antibody, clone 3B6.1
Alternate Names
  • Nephrin
  • Renal glomerulus-specific cell adhesion receptor
Background InformationNephrin (UniProt O60500; also known as Renal glomerulus-specific cell adhesion receptor) is encoded by the NPHS1 (also known as NPHN) gene (Gene ID 4868) in human. The transmembrane protein nephrin is an essential component of the podocyte slit diaphragm, the main functional unit of the glomerulus. The renal filtration barrier consists of fenestrated endothelial cells, the glomerular basement membrane (GBM), and the podocytes of epithelial cells. The interdigitating podocyte foot processes are bridged by a 40 nm wide extracellular structure known as the slit diaphragm. Together with the capillary endothelium and GBM, podocytes comprise the glomerular filtration barrier. The GBM functions as a coarse prefilter for large plasma proteins, whereas the slit diaphragm serves as an isoporous ultrafilter with pores smaller than albumin. Nephrin gene mutations are linked to the autosomal recessive disorder congenital nephrotic syndrome of the Finnish type (NPHS1) characterized by massive proteinuria already in utero and nephrosis soon after birth. Both nephrin and the slit diaphragm are reported to be absent in NPHS1 cases. Nephrin is produced with a signal peptide (a.a. 1-22), the removal of which yields the mature protein with a large extracellular region (a.a. 23-1055), followed by a transmembrane segment (a.a. 1056-1076) and a cytoplasmic tail (a.a. 1077-1241). The extracellular region contains eight C-type Ig-like domains (a.a. 27-939), and a Fibronectin type III domain (a.a. 943-1038), while the cytoplasmic tail contains the NPHS2-binding region (a.a. 1160-1241).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nephrin Antibody, clone 3B6.1 is an antibody against Nephrin for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to the fifth C2-type Ig-like domain of human Nephrin.
EpitopeC2-type Ig-like domain 5.
Clone3B6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3B6.1 recognizes an extracellular epitope in the fifth C2-type Ig-like domain that is present in both spliced isoforms of human nephrin reported by UniProt (O60500).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NPHS1
  • NPHN
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~185 kDa observed. Target band size appears larger than the calculated molecular weights of 132.5 kDa (isoform 1) and 128.3 kDa (isoform 2; isoform Alpha) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Nephrin in 10 µg of human kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABT416 04055977335446