Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF215 Anti-NOD1/CARD4 Antibody, clone 021

Anti-NOD1/CARD4 Antibody, clone 021 is an antibody against NOD1/CARD4 for use in western blotting & flow cytometry.

Anti-NOD1/CARD4 Antibody, clone 021 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: NOD1, CARD4 UniProt Number: Q9Y239
MABF215
100 µg  
Purchase on Sigma-Aldrich

Offres spéciales

Aperçu

Replacement Information

Offres spéciales

100% Performance Guaranteed

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF215
DescriptionAnti-NOD1/CARD4 Antibody, clone 021
Alternate Names
  • Nucleotide-binding oligomerization domain-containing protein 1
  • Caspase recruitment domain-containing protein 4
Background InformationNOD1/CARD4, also known as Nucleotide-binding oligomerization domain-containing protein 1, or Caspase recruitment domain-containing protein 4, and encoded by the gene NOD1/CARD4, is an important apoptosis mediating protein that enhances caspase-9-mediated apoptosis. NOD1/CARD4 also mediates cellular responsiveness to intracellular bacterial LPS and forms an intracellular sensing system along with another protein called ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. NOD1/CARD4 is a homodimer that is found in the cytoplasm but also associated with the cell membrane in particular cells such as polarized epithelial cells. NOD1/CARD4 is expressed in many tissues including heart, skeletal muscle, and pancreas. As part of the innate immune system NOD1/CARD4 is part of a family of proteins that are responsible for the initial response of an organism to potentially harmful stressors, pathogens or tissue injury, and accordingly plays an essential role in the pathogenesis of many inflammatory processes, including some cardiovascular diseases.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NOD1/CARD4 Antibody, clone 021 is an antibody against NOD1/CARD4 for use in western blotting & flow cytometry.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 2 µg of this antibody from a representative lot detected NOD1/CARD4 in 1X10E6 Jurkat cells. This antibody was produced in collaboration with the Scripps Research Institute.
Biological Information
ImmunogenRecombinant protein corresponding to the CARD domain of human NOD1/CARD4.
EpitopeCARD domain
Clone021
Concentration1 mg/mL
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOD1
  • CARD4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~125 kDa observed. The calculated molecular weight of this protein is 108 kDa, but has been observed at ~125 kDa in some cell lysates. An IgG heavy chain may be observed at 55 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluted by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NOD1/CARD4 in 10 µg of human spleen tissue lysate.

This antibody was produced in collaboration with the Scripps Research Institute. For Scripps Research Institute Validation (Click Here)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABF215 04053252977671